• Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (50 uL)
  • Sensitivity
    13.9 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Natural and recombinant human FGF acidic
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC22, Quantikine Immunoassay Control Group 7 - Please Inquire
Product Summary
The Quantikine Human FGF acidic Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FGF acidic in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human FGF acidic and has been shown to accurately quantitate the recombinant factor.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean179441749178433713
Standard Deviation8.331.817.61536.361
CV%4.67.22.38.48.48.6

Cell Culture Supernates, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean208485766204477761
Standard Deviation7.621.822.91529.348.7
CV%3.74.537.46.16.4

Recovery

The recovery of FGF acidic spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 105 93-113
EDTA Plasma (n=4) 102 93-109
Heparin Plasma (n=4) 97 92-110
Serum (n=4) 98 89-108
Urine (n=4) 99 89-108
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of FGF acidic were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human FGF acidic Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FGF acidic
FGF acidic, also known as FGF-1, ECGF, and HBGF-1, is a secreted mitogen that stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF acidic is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Internalized FGF acidic can translocate to the cytosol and to the nucleus. Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.
    • Long Name
      Fibroblast Growth Factor acidic
    • Entrez Gene IDs
      2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine);
    • Alternate Names
      AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF1; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 150 µL Assay Diluent
    4.   Add 150 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    6 Citations: Showing 1 - 6
    Filter your results:

    Species
    Sample Type
    1. Individuality in FGF1 expression significantly influences platinum resistance and progression-free survival in ovarian cancer.
      Br. J. Cancer, 2012;107(8):1327-36.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
      Authors: Herrmann S, Green J, Weber T, Scharfenberger A
      Bone, 2006;39(5):1156-63.
      Species: Human
      Sample Type: Tissue Homogenates
    3. Humoral and cellular factors responsible for coronary collateral formation.
      Authors: Sherman JA, Hall A, Malenka DJ, De Muinck ED, Simons M
      Am. J. Cardiol., 2006;98(9):1194-7.
      Species: Human
      Sample Type: Plasma
    4. Functional analysis of the chemokine receptor CCR3 on airway epithelial cells.
      Authors: Beck LA, Tancowny B, Brummet ME, Asaki SY, Curry SL, Penno MB, Foster M, Bahl A, Stellato C
      J. Immunol., 2006;177(5):3344-54.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Alpha-tocopheryl succinate inhibits malignant mesothelioma by disrupting the fibroblast growth factor autocrine loop: mechanism and the role of oxidative stress.
      Authors: Stapelberg M, Gellert N, Swettenham E, Tomasetti M, Witting PK, Procopio A, Neuzil J
      J. Biol. Chem., 2005;280(27):25369-76.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
      Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
      J. Immunol., 2005;175(9):6177-89.
      Species: Human
      Sample Type: Cell Culture Supernates
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Group 7

    Ctrl QC22
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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    Average Rating: 4.5 (Based on 2 reviews)

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