Detection of Fibronectin in WS‑1 Human Cell Line by Flow Cytometry.
WS‑1 human fetal skin fibroblast cell line was stained with Mouse Anti-Human Fibronectin APC‑conjugated Monoclonal Antibody (Catalog # IC1918A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Fibronectin is an extracellular matrix component that exists in
different alternately spliced isoforms. Fibronectin mediates cell
adhesion in its insoluble state but not as a soluble molecule.
Fibronectins play a role in cell adhesion, migration, differentiation,
and specific gene expression.
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