Human Fibronectin APC-conjugated Antibody

Catalog #: IC1918A Datasheet / COA / SDS

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IC1918A

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Detection of Fibronectin in HepG2 cells by Flow Cytometry

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Human Fibronectin APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Fibronectin in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # P1H11

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human plasma-derived Fibronectin

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

HepG2 huan hepatocellular carcinoma cell line or

WS‑1 human fetal skin fibroblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Fibronectin in HepG2 cells by Flow Cytometry HepG2 (filled histogram) and Jurkat (open histogram) cells were stained with Mouse Anti-Human Fibronectin APC‑conjugated Monoclonal Antibody (Catalog # IC1918A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry

Detection of Fibronectin antibody in WS‑1 Human Cell Line antibody by Flow Cytometry.

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Detection of Fibronectin in WS‑1 Human Cell Line by Flow Cytometry. WS-1 human fetal skin fibroblast cell line was stained with Mouse Anti-Human Fibronectin APC-conjugated Monoclonal Antibody (Catalog # IC1918A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fibronectin

Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.

Entrez Gene IDs

2335 (Human); 280794 (Bovine)

Alternate Names

CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF