Human Fibronectin Antibody
Human Fibronectin Antibody Summary
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Fibronectin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Fibronectin at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Fibronectin in HepG2 and Daudi Human Cell Lines. Fibronectin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining; left panel) and Daudi human Burkitt's lymphoma cell line (negative staining; right panel) using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Fibronectin in Human Breast Cancer Tissue. Fibronectin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue and fibroblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human Fibronectin by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Fibronectin at approximately 285 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918). The 12-230kDa separation system and EZ Standard Pack 5 are recommended for detecting human Fibronectin using Simple WesternTM.
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Detection of Human Fibronectin by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Fibronectin at approximately 230 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918).The 12-230kDa separation system and EZ Standard Pack 5 are recommended for detecting human Fibronectin using Simple WesternTM.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fibronectin
Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.
Product Datasheets
Citations for Human Fibronectin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Pharmacologic Targeting of MMP2/9 Decreases Peritoneal Metastasis Formation of Colorectal Cancer in a Human Ex Vivo Peritoneum Culture Model
Authors: J Koch, D Mönch, A Maa beta, A Mangold, M Gužvi?, T Mürdter, T Leibold, MH Dahlke, P Renner
Cancers, 2022;14(15):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Diabetes Aggravates Post-ischaemic Renal Fibrosis through Persistent Activation of TGF-?1 and Shh Signalling
Authors: DJ Kim, JM Kang, SH Park, HK Kwon, SJ Song, H Moon, SM Kim, JW Seo, YH Lee, YG Kim, JY Moon, SY Lee, Y Son, SH Lee
Sci Rep, 2017;7(1):16782.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
PDL regeneration via cell homing in delayed replantation of avulsed teeth.
Authors: Zhu W, Zhang Q, Zhang Y, Cen L, Wang J
J Transl Med, 2015;13(0):357.
Species: Canine
Sample Types: Whole Tissue
Applications: IHC-P -
Granzyme B releases vascular endothelial growth factor from extracellular matrix and induces vascular permeability.
Authors: Hendel, Alon, Hsu, Ivy, Granville, David J
Lab Invest, 2014;94(7):716-25.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
CD29 expressed on plasma cells is activated by divalent cations and soluble CD106 contained in the bone marrow plasma: refractory activation is associated with enhanced proliferation and exit of clonal plasma cells to circulation in multiple myeloma patients.
Authors: Martinez-Vinambres E, Garcia-Trujillo JA, Rodriguez-Martin E, Villar LM, Coll J, Roldan E
Leukemia, 2011;26(5):1098-105.
Species: Human
Sample Types: Bone Marrow Fluid
Applications: Neutralization -
Snail involves in the transforming growth factor beta1-mediated epithelial-mesenchymal transition of retinal pigment epithelial cells.
Authors: Li H, Wang H, Wang F, Gu Q, Xu X
PLoS ONE, 2011;6(8):e23322.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Characterization and functional analysis of osteoblast-derived fibulins in the human hematopoietic stem cell niche.
Authors: Hergeth SP, Aicher WK, Essl M, Schreiber TD, Sasaki T, Klein G
Exp. Hematol., 2008;36(8):1022-34.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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