Human Fibronectin Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Fibronectin in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # P1H11
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human plasma-derived Fibronectin
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Human Fibronectin (Catalog # 1918-FN)
  • Simple Western
    20 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Immunoprecipitation
    Yu, H. et al. (2004) Mol. Carcinog. 41:85.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Fibronectin in Human Breast Cancer Tissue. Fibronectin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue and fibroblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Fibronectin by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Fibronectin at approximately 285 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fibronectin
Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.
  • Entrez Gene IDs:
    2335 (Human); 280794 (Bovine)
  • Alternate Names:
    Fibronectin; FN1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. PDL regeneration via cell homing in delayed replantation of avulsed teeth.
    Authors: Zhu W, Zhang Q, Zhang Y, Cen L, Wang J
    J Transl Med, 2015;13(0):357.
    Species: Canine
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  2. Granzyme B releases vascular endothelial growth factor from extracellular matrix and induces vascular permeability.
    Authors: Hendel, Alon, Hsu, Ivy, Granville, David J
    Lab Invest, 2014;94(7):716-25.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. CD29 expressed on plasma cells is activated by divalent cations and soluble CD106 contained in the bone marrow plasma: refractory activation is associated with enhanced proliferation and exit of clonal plasma cells to circulation in multiple myeloma patients.
    Authors: Martinez-Vinambres E, Garcia-Trujillo JA, Rodriguez-Martin E, Villar LM, Coll J, Roldan E
    Leukemia, 2011;26(5):1098-105.
    Species: Human
    Sample Type: Bone Marrow Plasma
    Application: Neut
  4. Snail involves in the transforming growth factor beta1-mediated epithelial-mesenchymal transition of retinal pigment epithelial cells.
    Authors: Li H, Wang H, Wang F, Gu Q, Xu X
    PLoS ONE, 2011;6(8):e23322.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Characterization and functional analysis of osteoblast-derived fibulins in the human hematopoietic stem cell niche.
    Authors: Hergeth SP, Aicher WK, Essl M, Schreiber TD, Sasaki T, Klein G
    Exp. Hematol., 2008;36(8):1022-34.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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