Human Fibronectin PE-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Fibronectin in HepG2 Human Cell Line by Flow Cytometry. HepG2 human liver cell line was stained with Mouse Anti-Human Fibronectin PE-conjugated Monoclonal Antibody (Catalog # IC1918P, filled histogram) or isotype control antibody (IC002, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
Detection of Fibronectin in WS‑1 Human Cell Line by Flow Cytometry. WS-1 human fetal skin fibroblast cell line was stained with Mouse Anti-Human Fibronectin PE-conjugated Monoclonal Antibody (Catalog # IC1918P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.
Citation for Human Fibronectin PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Fibronectin on the Surface of Myeloma Cell-derived Exosomes Mediates Exosome-Cell Interactions.
Authors: Purushothaman A, Bandari S, Liu J, Mobley J, Brown E, Sanderson R
J Biol Chem, 2016;291(4):1652-63.
Sample Types: Whole Cells
Applications: Flow Cytometry
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