Human FoxJ1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human FoxJ1 in direct ELISAs.
  • Source
    Monoclonal Rat IgG2B Clone # 407003
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human FoxJ1
    Lys306-Leu421
    Accession # Q92949.3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    Immersion fixed HEK293 human embryonic kidney cell line
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of FoxJ1 in HEK293 Human Cell Line by Flow Cytometry. HEK293 human embryonic kidney cell line was stained with Rat Anti-Human FoxJ1 Monoclonal Antibody (Catalog # MAB3619, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxJ1

Human FoxJ1 (also HNF-4) is a 45 kDa, class 1 member of the HNF-3/fork-head gene family of transcription factors. It is 421 amino acids (aa) in length and contains one fork-head DNA binding domain (aa 120‑210). The presence of basic residues in the fork-head domain makes FoxJ1 a class 1 Fox protein. FoxJ1 induces cilia in lung and oviduct and maintains T cell tolerance to self-antigens. It is known to modulate NF kappa B activity. Over the range of amino acids used for immunization, human FoxJ1 is 89% aa identical to dog FoxJ1 and 87% aa identical to both mouse and rat FoxJ1.

  • Long Name:
    Forkhead Box J1
  • Entrez Gene IDs:
    2302 (Human); 15223 (Mouse); 116557 (Rat)
  • Alternate Names:
    FKHL13; FKHL13forkhead transcription factor HFH-4; forkhead box J1; forkhead-like 13; Forkhead-related protein FKHL13; FoxJ1; Hepatocyte nuclear factor 3 forkhead homolog 4; HFH-4; HFH4fork head homologue 4; HFH-4forkhead box protein J1; MGC35202
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