Human FoxJ1 Antibody

Catalog # Availability Size / Price Qty
MAB3619
MAB3619-SP
Detection of FoxJ1 in HEK293 Human Cell Line by Flow Cytometry.
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Human FoxJ1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FoxJ1 in direct ELISAs.
Source
Monoclonal Rat IgG2B Clone # 407003
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FoxJ1
Lys306-Leu421
Accession # Q92949.3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of FoxJ1 antibody in HEK293 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of FoxJ1 in HEK293 Human Cell Line by Flow Cytometry. HEK293 human embryonic kidney cell line was stained with Rat Anti-Human FoxJ1 Monoclonal Antibody (Catalog # MAB3619, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxJ1

Human FoxJ1 (also HNF-4) is a 45 kDa, class 1 member of the HNF-3/fork-head gene family of transcription factors. It is 421 amino acids (aa) in length and contains one fork-head DNA binding domain (aa 120‑210). The presence of basic residues in the fork-head domain makes FoxJ1 a class 1 Fox protein. FoxJ1 induces cilia in lung and oviduct and maintains T cell tolerance to self-antigens. It is known to modulate NF kappa B activity. Over the range of amino acids used for immunization, human FoxJ1 is 89% aa identical to dog FoxJ1 and 87% aa identical to both mouse and rat FoxJ1.

Long Name
Forkhead Box J1
Entrez Gene IDs
2302 (Human); 15223 (Mouse); 116557 (Rat)
Alternate Names
FKHL13; FKHL13forkhead transcription factor HFH-4; forkhead box J1; forkhead-like 13; Forkhead-related protein FKHL13; FoxJ1; Hepatocyte nuclear factor 3 forkhead homolog 4; HFH-4; HFH4fork head homologue 4; HFH-4forkhead box protein J1; MGC35202

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