Human Free BDNF Quantikine ELISA Kit

  (39 citations)     
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Product Details
Assay Procedure
Citations (39)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor Heparin Plasma (50 uL), Citrate Plasma (50 uL)
  • Sensitivity
    20 pg/mL
  • Assay Range
    62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human BDNF
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC22 , Quantikine Immunoassay Control Group 7 - Please Inquire
Product Summary
The Quantikine Human BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure human BDNF in cell culture supernates, serum, and plasma. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural human BDNF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human BDNF.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 334 825 1339 358 905 1517
Standard Deviation 15.6 28.3 56.5 36.8 86.8 121.6
CV% 4.7 3.4 4.2 10.3 9.6 8.1

Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 476 1258 2175 528 1338 2287
Standard Deviation 24 48.2 135.7 59.7 102.3 186
CV% 5 3.8 6.2 11.3 7.6 8.1

Recovery

The recovery of BDNF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 96 83-105
Platelet-poor Citrate Plasma (n=5) 99 87-120
Platelet-poor EDTA Plasma (n=5) 96 89-115
Platelet-poor Heparin Plasma (n=5) 93 81-119
Serum (n=5) 100 85-116
Linearity
To assess the linearity of the assay, five samples containing or spiked with high concentrations of BDNF in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 BDNF [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BDNF
BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.
  • Long Name:
    Brain-derived Neurotrophic Factor
  • Entrez Gene IDs:
    627 (Human); 12064 (Mouse); 24225 (Rat)
  • Alternate Names:
    Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; neurotrophin
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    Go to the next step. Do not wash.

  7. 100 µL Conjugate
  8.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  9.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  10. 200 µL Substrate Solution
  11.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  12. 50 µL Stop Solution
  13.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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Species
Sample Type
  1. Effects and mechanism of the HECT study (hybrid exercise-cognitive trainings) in mild ischemic stroke with cognitive decline: fMRI for brain plasticity, biomarker and behavioral analysis
    Authors: TT Yeh, KC Chang, CY Wu, YY Lee, PY Chen, JW Hung
    Contemp Clin Trials Commun, 2018;9(0):164-171.
    Species: Human
    Sample Type: Serum
  2. Engineered 3D vascular and neuronal networks in a microfluidic platform
    Authors: T Osaki, V Sivathanu, RD Kamm
    Sci Rep, 2018;8(1):5168.
    Species: Human
    Sample Type: Cell Culture Supernates
  3. Physical Exercise and Spatial Training: A Longitudinal Study of Effects on Cognition, Growth Factors, and Hippocampal Plasticity
    Authors: L Woost, PL Bazin, M Taubert, R Trampel, CL Tardif, A Garthe, G Kempermann, U Renner, G Stalla, DVM Ott, V Rjosk, H Obrig, A Villringer, E Roggenhofe, TA Klein
    Sci Rep, 2018;8(1):4239.
    Species: Human
    Sample Type: Serum
  4. Potential benefits of mesenchymal stem cells and electroacupuncture on the trophic factors associated with neurogenesis in mice with ischemic stroke
    Authors: YR Kim, SM Ahn, ME Pak, HJ Lee, DH Jung, YI Shin, HK Shin, BT Choi
    Sci Rep, 2018;8(1):2044.
    Species: Mouse
    Sample Type: Tissue Homogenates
  5. Serum nerve growth factor beta, brain- and glial-derived neurotrophic factor levels and psychopathology in unmedicated patients with schizophrenia
    Authors: CS Chu, CL Chu, CC Wu, T Lu
    J Chin Med Assoc, 2018;0(0):.
    Species: Human
    Sample Type: Serum
  6. The serum protein levels of the tPA-BDNF pathway are implicated in depression and antidepressant treatment
    Authors: H Jiang, S Chen, C Li, N Lu, Y Yue, Y Yin, Y Zhang, X Zhi, D Zhang, Y Yuan
    Transl Psychiatry, 2017;7(4):e1079.
    Species: Human
    Sample Type: Serum
  7. The Effects of Taekwondo Training on Peripheral Neuroplasticity-Related Growth Factors, Cerebral Blood Flow Velocity, and Cognitive Functions in Healthy Children: A Randomized Controlled Trial
    Authors: SY Cho, WY So, HT Roh
    Int J Environ Res Public Health, 2017;14(5):.
    Species: Human
    Sample Type: Serum
  8. Affective alterations in patients with Cushing's syndrome in remission are associated with decreased BDNF and cortisone levels
    Authors: E Valassi
    Eur. J. Endocrinol, 2017;176(2):221-231.
    Species: Human
    Sample Type: Serum
  9. A noradrenergic lesion aggravates the effects of systemic inflammation on the hippocampus of aged rats
    Authors: KL Bharani, R Derex, AC Granholm, A Ledreux
    PLoS ONE, 2017;12(12):e0189821.
    Species: Rat
    Sample Type: Serum
  10. Combined serum levels of multiple proteins in tPA-BDNF pathway may aid the diagnosis of five mental disorders
    Authors: S Chen, H Jiang, Y Liu, Z Hou, Y Yue, Y Zhang, F Zhao, Z Xu, Y Li, X Mou, L Li, T Wang, J Zhao, C Han, Y Sui, M Wang, Z Yang, Y Lu, Y Zhu, J Li, X Shen, F Sun, Q Chen, H Chen, Y Yuan
    Sci Rep, 2017;7(1):6871.
    Species: Human
    Sample Type: Serum
  11. Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
    Sci Rep, 2016;6(0):29029.
    Species: Human
    Sample Type: Cell Culture Supernates
  12. Cross-sectional associations of objectively-measured physical activity with brain-derived neurotrophic factor in adolescents
    Authors: Tao Huang
    Physiol. Behav, 2016;0(0):.
    Species: Human
    Sample Type: Serum
  13. Stress-induced change in serum BDNF is related to quantitative family history of alcohol use disorder and age at first alcohol use
    Authors: Shobhit Sharma
    Pharmacol. Biochem. Behav, 2016;153(0):12-17.
    Species: Human
    Sample Type: Serum
  14. Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
    Stem Cells Int, 2016;2016(0):6104780.
    Species: Human
    Sample Type: Cell Culture Supernates
  15. Exploring the Association between Serum BDNF and Attempted Suicide
    Sci Rep, 2016;6(0):25229.
    Species: Human
    Sample Type: Serum
  16. Serum brain-derived neurotrophic factor levels and personality traits in patients with major depression.
    Authors: Nomoto H, Baba H, Satomura E, Maeshima H, Takebayashi N, Namekawa Y, Suzuki T, Arai H
    BMC Psychiatry, 2015;15(0):33.
    Species: Human
    Sample Type: Serum
  17. Effects of exercise and milk fat globule membrane (MFGM) supplementation on body composition, physical function, and hematological parameters in community-dwelling frail Japanese women: a randomized double blind, placebo-controlled, follow-up trial.
    Authors: Kim H, Suzuki T, Kim M, Kojima N, Ota N, Shimotoyodome A, Hase T, Hosoi E, Yoshida H
    PLoS ONE, 2015;10(2):e0116256.
    Species: Human
    Sample Type: Serum
  18. The BDNF Val66Met polymorphism and plasma brain-derived neurotrophic factor levels in Han Chinese heroin-dependent patients.
    Authors: Chen, Shiou-La, Lee, Sheng-Yu, Chang, Yun-Hsua, Wang, Tzu-Yun, Chen, Shih-Hen, Chu, Chun-Hsi, Chen, Po See, Yang, Yen Kuan, Hong, Jau-Shyo, Lu, Ru-Band
    Sci Rep, 2015;5(0):8148.
    Species: Human
    Sample Type: Plasma
  19. Measurement of the levels of leptin, BDNF associated with polymorphisms LEP G2548A, LEPR Gln223Arg and BDNF Val66Met in Thai with metabolic syndrome.
    Authors: Suriyaprom, Kanjana, Tungtrongchitr, Rungsunn, Thawnasom, Kittisak
    Diabetol Metab Syndr, 2014;6(1):6.
    Species: Human
    Sample Type: Serum
  20. Brain-derived neurotrophic factor in human subjects with function-altering melanocortin-4 receptor variants.
    Authors: Hohenadel M, Thearle M, Grice B, Huang H, Dai M, Tao Y, Hunter L, Palaguachi G, Mou Z, Kim R, Tsang M, Haack K, Voruganti V, Cole S, Butte N, Comuzzie A, Muller Y, Baier L, Krakoff J, Knowler W, Yanovski J, Han J
    Int J Obes (Lond), 2014;38(8):1068-74.
    Species: Human
    Sample Type: Serum
  21. Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate.
    Authors: Chou M, Burnouf T, Wang T
    PLoS ONE, 2014;9(6):e99145.
    Species: Human
    Sample Type: Cell Culture Supernates
  22. Serum BDNF levels in relation to illness severity, suicide attempts, and central serotonin activity in patients with major depressive disorder: a pilot study.
    Authors: Park Y, Lee B, Um T, Kim S
    PLoS ONE, 2014;9(3):e91061.
    Species: Human
    Sample Type: Serum
  23. Bortezomib-induced peripheral neuropathy is related to altered levels of brain-derived neurotrophic factor in the peripheral blood of patients with multiple myeloma.
    Authors: Azoulay, David, Lavie, David, Horowitz, Netanel, Suriu, Celia, Gatt, Moshe E, Akria, Luiza, Perlman, Riki, Braester, Andrei, Ben-Yehuda, Dina
    Br J Haematol, 2014;164(3):454-6.
    Species: Human
    Sample Type: Serum
  24. A longitudinal study of alterations of hippocampal volumes and serum BDNF levels in association to atypical antipsychotics in a sample of first-episode patients with schizophrenia.
    Authors: Rizos E, Papathanasiou M, Michalopoulou P, Laskos E, Mazioti A, Kastania A, Vasilopoulou K, Nikolaidou P, Margaritis D, Papageorgiou C, Liappas I
    PLoS ONE, 2014;9(2):e87997.
    Species: Human
    Sample Type: Serum
  25. A randomized controlled trial of multicomponent exercise in older adults with mild cognitive impairment.
    Authors: Suzuki T, Shimada H, Makizako H, Doi T, Yoshida D, Ito K, Shimokata H, Washimi Y, Endo H, Kato T
    PLoS ONE, 2013;8(4):e61483.
    Species: Human
    Sample Type: Serum
  26. Brain-derived neurotrophic factor is increased in serum and skin levels of patients with chronic spontaneous urticaria.
    Authors: Rossing K, Novak N, Mommert S, Pfab F, Gehring M, Wedi B, Kapp A, Raap U
    Clin. Exp. Allergy, 2011;41(10):1392-1399.
    Species: Human
    Sample Type: Serum
  27. Exercise training increases size of hippocampus and improves memory.
    Authors: Erickson KI, Voss MW, Prakash RS
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(7):3017-22.
    Species: Human
    Sample Type: Serum
  28. CD34+ progenitor cells likely are involved in the good functional recovery after intracerebral hemorrhage in humans.
    Authors: Sobrino T, Arias S, Perez-Mato M, Agulla J, Brea D, Rodriguez-Yanez M, Castillo J
    J. Neurosci. Res., 2011;89(7):979-85.
    Species: Human
    Sample Type: Serum
  29. BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
    Authors: Huang YT, Lai PC, Wu CC, Hsu SH, Cheng CC, Lan YF, Chiu TH
    Int. J. Oncol., 2010;36(6):1469-76.
    Species: Human
    Sample Type: Cell Culture Supernates
  30. Androgen regulates brain-derived neurotrophic factor in spinal motoneurons and their target musculature.
    Authors: Verhovshek T, Cai Y, Osborne MC, Sengelaub DR
    Endocrinology, 2010;151(1):253-61.
    Species: Rat
    Sample Type: Tissue Homogenates
  31. Endurance training enhances BDNF release from the human brain.
    Authors: Seifert T, Brassard P, Wissenberg M, Rasmussen P, Nordby P, Stallknecht B, Adser H, Jakobsen AH, Pilegaard H, Nielsen HB, Secher NH
    Am. J. Physiol. Regul. Integr. Comp. Physiol., 2010;298(2):R372-7.
    Species: Human
    Sample Type: Plasma
  32. Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis.
    Authors: Sung SY, Hsieh CL, Law A, Zhau HE, Pathak S, Multani AS, Lim S, Coleman IM, Wu LC, Figg WD, Dahut WL, Nelson P, Lee JK, Amin MB, Lyles R, Johnstone PA, Marshall FF, Chung LW
    Cancer Res., 2008;68(23):9996-10003.
    Species: Human
    Sample Type: Serum
  33. Spinal adenosine A2a receptor activation elicits long-lasting phrenic motor facilitation.
    Authors: Golder FJ, Ranganathan L, Satriotomo I, Hoffman M, Lovett-Barr MR, Watters JJ, Baker-Herman TL, Mitchell GS
    J. Neurosci., 2008;28(9):2033-42.
    Species: Rat
    Sample Type: Tissue Homogenates
  34. Decreased brain-derived neurotrophic factor (BDNF)- and beta-thromboglobulin (beta-TG)- blood levels in Alzheimer's disease.
    Authors: Laske C, Stransky E, Leyhe T, Eschweiler GW, Schott K, Langer H, Gawaz M
    Thromb. Haemost., 2006;96(1):102-3.
    Species: Human
    Sample Type: Serum
  35. Intravenous administration of marrow stromal cells (MSCs) increases the expression of growth factors in rat brain after traumatic brain injury.
    Authors: Mahmood A, Lu D, Chopp M
    J. Neurotrauma, 2004;21(1):33-9.
    Species: Rat
    Sample Type: Tissue Homogenates
  36. Perinatal changes of brain-derived neurotrophic factor in pre- and fullterm neonates.
    Authors: Malamitsi-Puchner A, Economou E, Rigopoulou O, Boutsikou T
    Early Hum. Dev., 2004;76(1):17-22.
    Species: Human
    Sample Type: Serum
  37. Transcranial magnetic stimulation and BDNF plasma levels in amyotrophic lateral sclerosis.
    Authors: Angelucci F, Oliviero A, Pilato F, Saturno E, Dileone M, Versace V, Musumeci G, Batocchi AP, Tonali PA, Di Lazzaro V
    Neuroreport, 2004;15(4):717-20.
    Species: Human
    Sample Type: Serum
  38. AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression.
    Authors: Truckenmiller ME, Vawter MP, Zhang P, Conejero-Goldberg C, Dillon-Carter O, Morales N, Cheadle C, Becker KG, Freed WJ
    Exp. Neurol., 2002;175(0):318.
    Species: Rat
    Sample Type: Cell Culture Supernates
  39. Daily serum and salivary BDNF levels correlate with morning-evening personality type in women and are affected by light therapy.
    Authors: Tirassa P, Iannitelli A, Sornelli F, Cirulli F, Mazza M, Calza A, Alleva E, Branchi I, Aloe L, Bersani G, Pacitti F
    Riv Psichiatr, 0;47(6):527-34.
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