Human Free BDNF Quantikine ELISA Kit

(28 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor Heparin Plasma (50 uL), Citrate Plasma (50 uL)
  • Sensitivity
    20 pg/mL
  • Assay Range
    62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human BDNF
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC22, Quantikine Immunoassay Control Group 7 - Please Inquire
Product Summary
The Quantikine Human BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure human BDNF in cell culture supernates, serum, and plasma. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural human BDNF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human BDNF.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean4761258217552813382287
Standard Deviation2448.2135.759.7102.3186
CV%53.86.211.37.68.1

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean33482513393589051517
Standard Deviation15.628.356.536.886.8121.6
CV%4.73.44.210.39.68.1

Recovery

The recovery of BDNF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 96 83-105
Platelet-poor Citrate Plasma (n=5) 99 87-120
Platelet-poor EDTA Plasma (n=5) 96 89-115
Platelet-poor Heparin Plasma (n=5) 93 81-119
Serum (n=5) 100 85-116
Linearity
To assess the linearity of the assay, five samples containing or spiked with high concentrations of BDNF in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Free BDNF Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BDNF
BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.
    • Long Name
      Brain-derived Neurotrophic Factor
    • Entrez Gene IDs
      627 (Human); 12064 (Mouse); 24225 (Rat);
    • Alternate Names
      Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; neurotrophin;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
      Go to the next step. Do not wash.

    7. 100 µL Conjugate
    8.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
    9.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

    10. 200 µL Substrate Solution
    11.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    12. 50 µL Stop Solution
    13.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    28 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Combined serum levels of multiple proteins in tPA-BDNF pathway may aid the diagnosis of five mental disorders
      Authors: S Chen, H Jiang, Y Liu, Z Hou, Y Yue, Y Zhang, F Zhao, Z Xu, Y Li, X Mou, L Li, T Wang, J Zhao, C Han, Y Sui, M Wang, Z Yang, Y Lu, Y Zhu, J Li, X Shen, F Sun, Q Chen, H Chen, Y Yuan
      Sci Rep, 2017;7(1):6871.
      Species: Human
      Sample Type: Serum
    2. Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
      Stem Cells Int, 2016;2016(0):6104780.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. Exploring the Association between Serum BDNF and Attempted Suicide
      Sci Rep, 2016;6(0):25229.
      Species: Human
      Sample Type: Serum
    4. Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
      Sci Rep, 2016;6(0):29029.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Serum brain-derived neurotrophic factor levels and personality traits in patients with major depression.
      Authors: Nomoto H, Baba H, Satomura E, Maeshima H, Takebayashi N, Namekawa Y, Suzuki T, Arai H
      BMC Psychiatry, 2015;15(0):33.
      Species: Human
      Sample Type: Serum
    6. Effects of exercise and milk fat globule membrane (MFGM) supplementation on body composition, physical function, and hematological parameters in community-dwelling frail Japanese women: a randomized double blind, placebo-controlled, follow-up trial.
      Authors: Kim H, Suzuki T, Kim M, Kojima N, Ota N, Shimotoyodome A, Hase T, Hosoi E, Yoshida H
      PLoS ONE, 2015;10(2):e0116256.
      Species: Human
      Sample Type: Serum
    7. The BDNF Val66Met polymorphism and plasma brain-derived neurotrophic factor levels in Han Chinese heroin-dependent patients.
      Authors: Chen, Shiou-La, Lee, Sheng-Yu, Chang, Yun-Hsua, Wang, Tzu-Yun, Chen, Shih-Hen, Chu, Chun-Hsi, Chen, Po See, Yang, Yen Kuan, Hong, Jau-Shyo, Lu, Ru-Band
      Sci Rep, 2015;5(0):8148.
      Species: Human
      Sample Type: Plasma
    8. Bortezomib-induced peripheral neuropathy is related to altered levels of brain-derived neurotrophic factor in the peripheral blood of patients with multiple myeloma.
      Authors: Azoulay, David, Lavie, David, Horowitz, Netanel, Suriu, Celia, Gatt, Moshe E, Akria, Luiza, Perlman, Riki, Braester, Andrei, Ben-Yehuda, Dina
      Br J Haematol, 2014;164(3):454-6.
      Species: Human
      Sample Type: Serum
    9. A longitudinal study of alterations of hippocampal volumes and serum BDNF levels in association to atypical antipsychotics in a sample of first-episode patients with schizophrenia.
      Authors: Rizos E, Papathanasiou M, Michalopoulou P, Laskos E, Mazioti A, Kastania A, Vasilopoulou K, Nikolaidou P, Margaritis D, Papageorgiou C, Liappas I
      PLoS ONE, 2014;9(2):e87997.
      Species: Human
      Sample Type: Serum
    10. Brain-derived neurotrophic factor in human subjects with function-altering melanocortin-4 receptor variants.
      Authors: Hohenadel M, Thearle M, Grice B, Huang H, Dai M, Tao Y, Hunter L, Palaguachi G, Mou Z, Kim R, Tsang M, Haack K, Voruganti V, Cole S, Butte N, Comuzzie A, Muller Y, Baier L, Krakoff J, Knowler W, Yanovski J, Han J
      Int J Obes (Lond), 2014;38(8):1068-74.
      Species: Human
      Sample Type: Serum
    11. Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate.
      Authors: Chou M, Burnouf T, Wang T
      PLoS ONE, 2014;9(6):e99145.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Serum BDNF levels in relation to illness severity, suicide attempts, and central serotonin activity in patients with major depressive disorder: a pilot study.
      Authors: Park Y, Lee B, Um T, Kim S
      PLoS ONE, 2014;9(3):e91061.
      Species: Human
      Sample Type: Serum
    13. Measurement of the levels of leptin, BDNF associated with polymorphisms LEP G2548A, LEPR Gln223Arg and BDNF Val66Met in Thai with metabolic syndrome.
      Authors: Suriyaprom, Kanjana, Tungtrongchitr, Rungsunn, Thawnasom, Kittisak
      Diabetol Metab Syndr, 2014;6(1):6.
      Species: Human
      Sample Type: Serum
    14. A randomized controlled trial of multicomponent exercise in older adults with mild cognitive impairment.
      Authors: Suzuki T, Shimada H, Makizako H, Doi T, Yoshida D, Ito K, Shimokata H, Washimi Y, Endo H, Kato T
      PLoS ONE, 2013;8(4):e61483.
      Species: Human
      Sample Type: Serum
    15. Exercise training increases size of hippocampus and improves memory.
      Authors: Erickson KI, Voss MW, Prakash RS
      Proc. Natl. Acad. Sci. U.S.A., 2011;108(7):3017-22.
      Species: Human
      Sample Type: Serum
    16. Brain-derived neurotrophic factor is increased in serum and skin levels of patients with chronic spontaneous urticaria.
      Authors: Rossing K, Novak N, Mommert S, Pfab F, Gehring M, Wedi B, Kapp A, Raap U
      Clin. Exp. Allergy, 2011;41(10):1392-1399.
      Species: Human
      Sample Type: Serum
    17. CD34+ progenitor cells likely are involved in the good functional recovery after intracerebral hemorrhage in humans.
      Authors: Sobrino T, Arias S, Perez-Mato M, Agulla J, Brea D, Rodriguez-Yanez M, Castillo J
      J. Neurosci. Res., 2011;89(7):979-85.
      Species: Human
      Sample Type: Serum
    18. Androgen regulates brain-derived neurotrophic factor in spinal motoneurons and their target musculature.
      Authors: Verhovshek T, Cai Y, Osborne MC, Sengelaub DR
      Endocrinology, 2010;151(1):253-61.
      Species: Rat
      Sample Type: Tissue Homogenates
    19. Endurance training enhances BDNF release from the human brain.
      Authors: Seifert T, Brassard P, Wissenberg M, Rasmussen P, Nordby P, Stallknecht B, Adser H, Jakobsen AH, Pilegaard H, Nielsen HB, Secher NH
      Am. J. Physiol. Regul. Integr. Comp. Physiol., 2010;298(2):R372-7.
      Species: Human
      Sample Type: Plasma
    20. BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
      Authors: Huang YT, Lai PC, Wu CC, Hsu SH, Cheng CC, Lan YF, Chiu TH
      Int. J. Oncol., 2010;36(6):1469-76.
      Species: Human
      Sample Type: Cell Culture Supernates
    21. Spinal adenosine A2a receptor activation elicits long-lasting phrenic motor facilitation.
      Authors: Golder FJ, Ranganathan L, Satriotomo I, Hoffman M, Lovett-Barr MR, Watters JJ, Baker-Herman TL, Mitchell GS
      J. Neurosci., 2008;28(9):2033-42.
      Species: Rat
      Sample Type: Tissue Homogenates
    22. Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis.
      Authors: Sung SY, Hsieh CL, Law A, Zhau HE, Pathak S, Multani AS, Lim S, Coleman IM, Wu LC, Figg WD, Dahut WL, Nelson P, Lee JK, Amin MB, Lyles R, Johnstone PA, Marshall FF, Chung LW
      Cancer Res., 2008;68(23):9996-10003.
      Species: Human
      Sample Type: Serum
    23. Decreased brain-derived neurotrophic factor (BDNF)- and beta-thromboglobulin (beta-TG)- blood levels in Alzheimer's disease.
      Authors: Laske C, Stransky E, Leyhe T, Eschweiler GW, Schott K, Langer H, Gawaz M
      Thromb. Haemost., 2006;96(1):102-3.
      Species: Human
      Sample Type: Serum
    24. Intravenous administration of marrow stromal cells (MSCs) increases the expression of growth factors in rat brain after traumatic brain injury.
      Authors: Mahmood A, Lu D, Chopp M
      J. Neurotrauma, 2004;21(1):33-9.
      Species: Rat
      Sample Type: Tissue Homogenates
    25. Perinatal changes of brain-derived neurotrophic factor in pre- and fullterm neonates.
      Authors: Malamitsi-Puchner A, Economou E, Rigopoulou O, Boutsikou T
      Early Hum. Dev., 2004;76(1):17-22.
      Species: Human
      Sample Type: Serum
    26. Transcranial magnetic stimulation and BDNF plasma levels in amyotrophic lateral sclerosis.
      Authors: Angelucci F, Oliviero A, Pilato F, Saturno E, Dileone M, Versace V, Musumeci G, Batocchi AP, Tonali PA, Di Lazzaro V
      Neuroreport, 2004;15(4):717-20.
      Species: Human
      Sample Type: Serum
    27. AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression.
      Authors: Truckenmiller ME, Vawter MP, Zhang P, Conejero-Goldberg C, Dillon-Carter O, Morales N, Cheadle C, Becker KG, Freed WJ
      Exp. Neurol., 2002;175(0):318.
      Species: Rat
      Sample Type: Cell Culture Supernates
    28. Daily serum and salivary BDNF levels correlate with morning-evening personality type in women and are affected by light therapy.
      Authors: Tirassa P, Iannitelli A, Sornelli F, Cirulli F, Mazza M, Calza A, Alleva E, Branchi I, Aloe L, Bersani G, Pacitti F
      Riv Psichiatr, 0;47(6):527-34.
    Expand to show all 28 Citations
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