Human Fucosyltransferase 7/FUT7 Alexa Fluor™ Plus 555‑conjugated Antibody

R&D Systems | Catalog # FAB64091AFP555

R&D Systems
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 795116
View all Fucosyltransferase 7/FUT7 Primary Antibodies »

Product Specifications

Specificity

Detects human Fucosyltransferase 7/FUT7 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Fucosyltransferase 5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Fucosyltransferase 7/FUT7

N-glycans, O-glycans and glycolipids are frequently fucosylated at terminal sites. Therefore, fucose is often part of a sugar epitope with important biological function. Well known fucose containing glycans include Lewis and ABO blood group antigens. Lewis epitopes are key elements involved in the leukocyte homing and extravasation process and thus are important for lymphocyte maturation and natural defense functions. Fucose-containing glycans also play critical roles in cell signaling and development (1). More than 10 fucosyltransferases have been cloned (2). FUT1 and FUT2 are alpha 1-2 fucosyltransferases and are responsible for ABO blood-group antigen synthesis. FUT8 is an alpha 1-6 fucosyltransferase that adds a fucose to the chitobiose core of N-glycans (3). FUT3, FUT4, FUT5, FUT6, FUT7 and FUT9 are alpha 1-3 or alpha 1-4 fucosyltransferases and are responsible for Lewis antigen generation. FUT7 plays an exclusive role for the biosynthesis of sialyl Lewis X epitope (NeuAc alpha 2,3Gal beta 1,4[Fuc alpha 1,3]GlcNAc) that serves as a ligand in the E-selectin and P-selectin mediated adhesion of leukocytes to activated endothelium or platelets (4, 5, 6). Expression of FUT7 is increased by hypoxia in human colon cancer cell lines (7). Within aa 36-342, human FUT7 shares 84% aa sequence identity with mouse and rat FUT7.

References

  1. Jafar-Nejad, H. et al. (2010) Glycobiology 20:931.
  2. Becker, D.J. et al. (2003) Glycobiology 13:41R.
  3. Lee, S.H. et al. (2006) J. Biochem. 139:391.
  4. Blander, J. M. et al. (1999) J. Immunol. 163:3746.
  5. Natsuka, S. et al. (1994) J. Biol. Chem. 269:16789.
  6. Sasaki, K. et al. (1994) J. Biol. Chem. 269:14730.
  7. Koike, T. et al. (2004) P.N.A.S. 101:8132.

Alternate Names

FTVII, Fuc-TVII, FucT-VII, FUT7

Entrez Gene IDs

2529 (Human); 14347 (Mouse); 296564 (Rat)

Gene Symbol

FUT7

UniProt

Q11130 (Human)

Additional Fucosyltransferase 7/FUT7 Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Glycobiology-related Enzymes

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Protocols

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