Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 795116
View all Fucosyltransferase 7/FUT7 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Fucosyltransferase 7/FUT7
Ala36-Ala342
Accession # Q11130

Specificity

Detects human Fucosyltransferase 7/FUT7 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Fucosyltransferase 5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Fucosyltransferase 7/FUT7 Antibody

Detection of Human Fucosyltransferase 7/FUT7 antibody by Western Blot.

Detection of Human Fucosyltransferase 7/FUT7 by Western Blot.

Western blot shows lysates of HuT 78 human cutaneous T cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Fucosyltransferase 7/ FUT7 Monoclonal Antibody (Catalog # MAB64091) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fucosyltransferase 7/FUT7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Fucosyltransferase 7/FUT7 antibody in HL-60 Human Cell Line by Immunocytochemistry (ICC).

Fucosyltransferase 7/FUT7 in HL‑60 Human Cell Line.

Fucosyltransferase 7/FUT7 was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human Fucosyltransferase 7/FUT7 Monoclonal Antibody (Catalog # MAB64091) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Fucosyltransferase 7/FUT7 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HL‑60 human acute promyelocytic leukemia cell line

Western Blot

2 µg/mL
Sample: HuT 78 human cutaneous T cell lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fucosyltransferase 7/FUT7

N-glycans, O-glycans and glycolipids are frequently fucosylated at terminal sites. Therefore, fucose is often part of a sugar epitope with important biological function. Well known fucose containing glycans include Lewis and ABO blood group antigens. Lewis epitopes are key elements involved in the leukocyte homing and extravasation process and thus are important for lymphocyte maturation and natural defense functions. Fucose-containing glycans also play critical roles in cell signaling and development (1). More than 10 fucosyltransferases have been cloned (2). FUT1 and FUT2 are alpha 1-2 fucosyltransferases and are responsible for ABO blood-group antigen synthesis. FUT8 is an alpha 1-6 fucosyltransferase that adds a fucose to the chitobiose core of N-glycans (3). FUT3, FUT4, FUT5, FUT6, FUT7 and FUT9 are alpha 1-3 or alpha 1-4 fucosyltransferases and are responsible for Lewis antigen generation. FUT7 plays an exclusive role for the biosynthesis of sialyl Lewis X epitope (NeuAc alpha 2,3Gal beta 1,4[Fuc alpha 1,3]GlcNAc) that serves as a ligand in the E-selectin and P-selectin mediated adhesion of leukocytes to activated endothelium or platelets (4, 5, 6). Expression of FUT7 is increased by hypoxia in human colon cancer cell lines (7). Within aa 36-342, human FUT7 shares 84% aa sequence identity with mouse and rat FUT7.

References

  1. Jafar-Nejad, H. et al. (2010) Glycobiology 20:931.
  2. Becker, D.J. et al. (2003) Glycobiology 13:41R.
  3. Lee, S.H. et al. (2006) J. Biochem. 139:391.
  4. Blander, J. M. et al. (1999) J. Immunol. 163:3746.
  5. Natsuka, S. et al. (1994) J. Biol. Chem. 269:16789.
  6. Sasaki, K. et al. (1994) J. Biol. Chem. 269:14730.
  7. Koike, T. et al. (2004) P.N.A.S. 101:8132.

Alternate Names

FTVII, Fuc-TVII, FucT-VII, FUT7

Entrez Gene IDs

2529 (Human); 14347 (Mouse); 296564 (Rat)

Gene Symbol

FUT7

UniProt

Q11130 (Human)

Additional Fucosyltransferase 7/FUT7 Products

Product Documents for Human Fucosyltransferase 7/FUT7 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fucosyltransferase 7/FUT7 Antibody

For research use only

Related Research Areas

Glycobiology-related Enzymes

Citations for Human Fucosyltransferase 7/FUT7 Antibody

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Protocols

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