Human GAB1 Antibody

Name: Human GAB1 Antibody
Brand: R&D Systems
SKU: MAB7077

Catalog #: MAB7077 Datasheet / COA / SDS

Discontinued Product

MAB7077 has been discontinued.

View all products.

Detection of Human and Mouse GAB1 by Western Blot.

1 Image

Product Details

FAQs

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GAB1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GAB1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human GAB2, 3, or 4 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 709008

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GAB1
Asn81-Glu283
Accession # Q13480

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human and Mouse GAB1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GAB1 Monoclonal Antibody (Catalog # MAB7077) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for GAB1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background:

Gab1 (GRB2-associated-binding protein 1) is a 110 kDa member of the Gab family of scaffolding proteins. It is widely expressed, including in T and B cells, and serves as an adaptor molecule for the transmission of signals from cytokine and growth factor receptors. Tyrosine phosphorylation of Gab1 by multiple receptor tyrosine kinases, such as HGF R/c-Met, mediates interaction with multiple proteins containing SH2 domains, such as SHP-2. The 695 amino acid (aa) human Gab1 contains one pleckstrin homology domain (aa 5-116) and a proline-rich sequence (aa 449-540). One isoform with insertion of 30 aa after aa 528 is reported. Over aa 81-283, human Gab1 shares 81% and 82% aa identity with mouse and rat Gab1, respectively.