GABAA R beta 3 was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human GABAA R beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5590) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human GABAA R beta 3 by Western Blot.
Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GABAA R beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5590) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GABAA R beta 3 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human GABAA R beta 3 by Simple WesternTM.
Simple Western lane view shows lysates of human brain cerebellum tissue, loaded at 0.2 mg/mL. A specific band was detected for GABAA R beta 3 at approximately 78 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human GABAA R beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5590) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GABA-A R beta 3
GABAA R beta 3 (GABA-A receptor subunit beta 3; also GABR3) is a 53-58 kDa member of the ligand-gated ion channel family of proteins. It is generally one of three beta-type subunits that contribute to a GABA-binding pentameric, postsynaptic chloride ion channel on neurons. Alternatively, it can apparently form a homopentameric channel that does not respond to GABA. Mature human GABAA R beta 3 is 448 amino acids (aa) in length. It is a 4-transmembrane glycoprotein that contains an extended N-terminal extracellular region (aa 26-245) and a long cytoplasmic loop between aa 328-450. There are potential splice variants. One shows an alternate start site at Met65, while others contain either a 48 aa substitution for aa 1-228, a nine aa substitution for aa 1-80, or a 26 aa substitution for aa 1-26. Over aa 26-245, human and mouse GABAA R beta 3 are absolutely identical in aa sequence.
gamma-Aminobutyric Acid Type A Receptor, beta-3 Polypeptide
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