Human GABAA R  beta 3 Antibody

AF5590 has been discontinued. View all GABA-A R beta 3 products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human GABAA R beta 3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant rat GABAB R2 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human GABAA R beta 3
    Gln26-Tyr245
    Accession # P28472
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
GABAA R  beta 3 in Human Brain.

GABAA R  beta 3 was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human GABAA R  beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5590) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human GABAA R  beta 3 by Western Blot.

Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GABAA R  beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5590) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GABAA R  beta 3 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human GABAA R  beta 3 by Simple WesternTM. Simple Western lane view shows lysates of human brain cerebellum tissue, loaded at 0.2 mg/mL. A specific band was detected for GABAA R  beta 3 at approximately 78 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human GABAA R  beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5590) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GABA-A R beta 3

GABAA R beta 3 (GABA-A receptor subunit beta 3; also GABR3) is a 53-58 kDa member of the ligand-gated ion channel family of proteins. It is generally one of three beta-type subunits that contribute to a GABA-binding pentameric, postsynaptic chloride ion channel on neurons. Alternatively, it can apparently form a homopentameric channel that does not respond to GABA. Mature human GABAA R beta 3 is 448 amino acids (aa) in length. It is a 4-transmembrane glycoprotein that contains an extended N-terminal extracellular region (aa 26-245) and a long cytoplasmic loop between aa 328-450. There are potential splice variants. One shows an alternate start site at Met65, while others contain either a 48 aa substitution for aa 1-228, a nine aa substitution for aa 1-80, or a 26 aa substitution for aa 1-26. Over aa 26-245, human and mouse GABAA R beta 3 are absolutely identical in aa sequence.

  • Long Name:
    gamma-Aminobutyric Acid Type A Receptor, beta-3 Polypeptide
  • Entrez Gene IDs:
    2562 (Human); 14402 (Mouse); 24922 (Rat)
  • Alternate Names:
    ECA5; GABA A R beta 3; GABA(A) receptor beta-3 subunit; GABA(A) receptor subunit beta-3; GABAA receptor beta-3 subunit; GABA-alpha receptor beta-2 subunit; GABAAR beta 3; GABAARb3; GABRB3; gamma-aminobutyric acid (GABA) A receptor, beta 3; gamma-aminobutyric acid receptor subunit beta-3; MGC9051
Related Research Areas

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