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Human GABAA R  beta 3 Antibody

Catalog #: AF5590 Datasheet / COA / SDS

Discontinued Product

AF5590 has been discontinued.
View all GABA-A R beta 3 products.
Detection of Human GABAAR beta 3 by Western Blot.
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Human GABAA R  beta 3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GABAA R beta 3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant rat GABAB R2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GABAA R beta 3
Gln26-Tyr245
Accession # P28472
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human GABA<SUB>A</SUB>R beta 3 antibody by Western Blot. View Larger

Detection of Human GABAAR beta 3 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GABAAR beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5590) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GABAAR beta 3 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry GABA<sub>A</sub>R beta 3 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

GABAAR beta 3 in Human Brain. GABAAR beta 3 was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human GABAAR beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5590) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human GABA<sub>A</sub>R beta 3 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human GABAAR beta 3 by Simple WesternTM. Simple Western lane view shows lysates of human brain cerebellum tissue, loaded at 0.2 mg/mL. A specific band was detected for GABAAR beta 3 at approximately 78 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human GABAAR beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5590) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GABA-A R beta 3

GABAA R beta 3 (GABA-A receptor subunit beta 3; also GABR3) is a 53-58 kDa member of the ligand-gated ion channel family of proteins. It is generally one of three beta-type subunits that contribute to a GABA-binding pentameric, postsynaptic chloride ion channel on neurons. Alternatively, it can apparently form a homopentameric channel that does not respond to GABA. Mature human GABAA R beta 3 is 448 amino acids (aa) in length. It is a 4-transmembrane glycoprotein that contains an extended N-terminal extracellular region (aa 26-245) and a long cytoplasmic loop between aa 328-450. There are potential splice variants. One shows an alternate start site at Met65, while others contain either a 48 aa substitution for aa 1-228, a nine aa substitution for aa 1-80, or a 26 aa substitution for aa 1-26. Over aa 26-245, human and mouse GABAA R beta 3 are absolutely identical in aa sequence.

Long Name
gamma-Aminobutyric Acid Type A Receptor, beta-3 Polypeptide
Entrez Gene IDs
2562 (Human); 14402 (Mouse); 24922 (Rat)
Alternate Names
ECA5; GABA A R beta 3; GABA(A) receptor beta-3 subunit; GABA(A) receptor subunit beta-3; GABAA receptor beta-3 subunit; GABA-alpha receptor beta-2 subunit; GABAAR beta 3; GABAARb3; GABRB3; gamma-aminobutyric acid (GABA) A receptor, beta 3; gamma-aminobutyric acid receptor subunit beta-3; MGC9051

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