Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Polyclonal Goat IgG
View all GATA-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GATA-2
Ala15-Thr279
Accession # P23769

Specificity

Detects human GATA-2. In direct ELISAs and Western blots, this antibody shows less than 1% cross-reactivity with rhGATA-1, rhGATA-5 and rhGATA-6.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human GATA‑2 PE‑conjugated Antibody

Detection of GATA-2 antibody in TF-1 Human Cell Line antibody by Flow Cytometry.

Detection of GATA‑2 in TF‑1 Human Cell Line by Flow Cytometry.

TF-1 human erythroleukemic cell line was stained with Goat Anti-Human GATA-2 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2046P, filled histogram) or control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Intracellular Molecules.

Applications for Human GATA‑2 PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: TF‑1 human erythroleukemic cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: GATA-2

GATA factors constitute a family of transcriptional regulatory factors that bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA-1/2/3 are each expressed in the hematopoietic system while GATA 4/5/6 are each expressed in the developing heart and in gastrointestinal and gut-derived tissues (1, 2).

References

  1. Tsai, S.F. et al. (1989) Nature 339:446.
  2. Jiang, Y. and T. Evans (1996) Dev. Biol. 174:258.

Alternate Names

GATA2

Entrez Gene IDs

2624 (Human)

Gene Symbol

GATA2

UniProt

P23769

Additional GATA-2 Products

Product Documents for Human GATA‑2 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GATA‑2 PE‑conjugated Antibody

For research use only

Citations for Human GATA‑2 PE‑conjugated Antibody

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