Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GATA-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GATA-2
Ala15-Thr279
Accession # P23769

Specificity

Detects human and mouse GATA-2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse GATA‑2 Antibody

Detection of Human GATA-2 antibody by Western Blot.

Detection of Human GATA‑2 by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and KG-1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse GATA-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA-2 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human GATA-2 antibody by Western Blot.

Detection of Human GATA‑2 by Western Blot.

Western blot shows lysates of LNCaP human prostate cancer cell line and SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse GATA-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA-2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GATA‑2 in HUVEC Human Cells.

GATA‑2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human/Mouse GATA‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

GATA‑2 in SH‑SY5Y Human Cell Line.

GATA‑2 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human/Mouse GATA‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

GATA-2 antibody in Human Duodenum by Immunohistochemistry (IHC-P).

GATA‑2 in Human Duodenum.

GATA-2 was detected in immersion fixed paraffin-embedded sections of human duodenum (blood vessel) using Goat Anti-Human/Mouse GATA-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human GATA-2 antibody by Simple WesternTM.

Detection of Human GATA‑2 by Simple WesternTM.

Simple Western lane view shows lysates of KG-1 human acute myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for GATA-2 at approximately 64 kDa (as indicated) using 5 µg/mL of Goat Anti-Human GATA-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse GATA-2 by Western Blot

Detection of Mouse GATA-2 by Western Blot

RNA-seq identifies the targets of GATA2 in primary human LECs. (A) Principal component analysis (PCA) was performed on RNA-seq data from control shRNA- and shGATA2-infected primary HLECs. A high level of similarity was observed within the groups as indicated by their proximity to each other. (B) Hierarchical clustering shows that approximately 1000 genes were consistently downregulated and 600 genes were upregulated in shGATA2-treated HLECs. (C) GO revealed a list of genes that are likely relevant to the phenotypes observed in mice lacking GATA2. (D) GATA2 was knocked out from a second HLEC line using CRISPR/Cas9. Western blot revealed the lack of GATA2 in the knockout cells (HLEC delta GATA2). In contrast, no obvious differences were observed in the expression of PROX1. Additionally, qRT-PCR revealed the downregulation of miR-126. (A) n=3 independent experiments per shRNA; (D) n=3 independent experiments (antibiotic selection, western blot and qRT-PCR). **P<0.01. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31582413), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human GATA-2 by Western Blot

Detection of Human GATA-2 by Western Blot

Changes in KIT gene expression by GATA1 siRNAs. (a) qRT-PCR analysis was performed for K562-WT (left) and one of the K562-G1s clone, C1 #26 (right). The vertical axis represents a relative value with each value of non-target siRNA set at 1. Results are averages of three independent experiments. Error bars represent standard deviation. *P < 0.05, **P < 0.01 (two-side t-test). (b) Representative immunoblots of siRNA experiments with K562-WT (left) and G1s C1 #26 (right). Western blotting was performed with anti-GATA1 antibody (D24E4, Cell signaling technology), anti-GATA2 (AF2046, R&D) anti-c-Kit (SC-17806, Santa Cruz) and anti-beta -actin antibody (Sigma-Aldrich). Images were obtained using ChemiDoc MP. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36447001), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human GATA-2 by Western Blot

Detection of Human GATA-2 by Western Blot

Changes in KIT gene expression by GATA1 siRNAs. (a) qRT-PCR analysis was performed for K562-WT (left) and one of the K562-G1s clone, C1 #26 (right). The vertical axis represents a relative value with each value of non-target siRNA set at 1. Results are averages of three independent experiments. Error bars represent standard deviation. *P < 0.05, **P < 0.01 (two-side t-test). (b) Representative immunoblots of siRNA experiments with K562-WT (left) and G1s C1 #26 (right). Western blotting was performed with anti-GATA1 antibody (D24E4, Cell signaling technology), anti-GATA2 (AF2046, R&D) anti-c-Kit (SC-17806, Santa Cruz) and anti-beta -actin antibody (Sigma-Aldrich). Images were obtained using ChemiDoc MP. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36447001), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse GATA‑2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells  and SH‑SY5Y human neuroblastoma cell line

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human duodenum (blood vessel)

Simple Western

5 µg/mL
Sample: KG‑1 human acute myelogenous leukemia cell line

Western Blot

0.5 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line, KG-1 human acute myelogenous leukemia cell line, LNCaP human prostate cancer cell line, and SH‑SY5Y human neuroblastoma cell line

Reviewed Applications

Read 1 review rated 5 using AF2046 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GATA-2

GATA factors constitute a family of transcriptional regulatory factors that bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA-1/2/3 are each expressed in the hematopoietic system while GATA 4/5/6 are each expressed in the developing heart and in gastrointestinal and gut-derived tissues (1, 2).

References

  1. Tsai, S.F. et al. (1989) Nature 339:446.
  2. Jiang, Y. and T. Evans (1996) Dev. Biol. 174:258.

Alternate Names

GATA2

Entrez Gene IDs

2624 (Human)

Gene Symbol

GATA2

UniProt

P23769

Additional GATA-2 Products

Product Documents for Human/Mouse GATA‑2 Antibody

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Product Specific Notices for Human/Mouse GATA‑2 Antibody

For research use only

Citations for Human/Mouse GATA‑2 Antibody

Customer Reviews for Human/Mouse GATA‑2 Antibody (1)

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  • Human/Mouse GATA-2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HUVEC human umbilical vein endothelial cells and Blood progenitor cells
    Species: Human
    Verified Customer | Posted 05/17/2022
    Stained with AF+ secondary antibody on endothelial cells undergoing differentiation.
    Human/Mouse GATA‑2 Antibody AF2046

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