Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all GATA-5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GATA-5
Met1-Ala397
Accession # Q9BWX5

Specificity

Detects human GATA-5 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human (rh) GATA-1, rhGATA-2, rhGATA-3, rhGATA-4 and rhGATA-6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human GATA‑5 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human GATA‑5

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GATA-5

GATA-5 belongs to the GATA family of transcription factors which bind to the consensus DNA sequence (AIT) GATA (AIG) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA5 is important in the development and differentiation of endoderm-derived organs, including heart and gastrointestinal tissues. GATA-5 has also been implicated in cancer development (1, 2).

References

  1. Tsai, S.F. et al. (1989) Nature 339:446.
  2. Jiang, Y. and T. Evans (1996) Dev. Biol. 174:258.

Alternate Names

GATA5

Entrez Gene IDs

140628 (Human)

Gene Symbol

GATA5

UniProt

Q9BWX5

Additional GATA-5 Products

Product Documents for Human GATA‑5 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GATA‑5 Biotinylated Antibody

For research use only

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FAQs

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