Human GATA-5 Antibody

Detection of GATA-5 by Immunohistochemistry

Identification of GATA5 target genes in response to C6orf223-mediated CRC growth and metastasis.(A) Binding motif of GATA5 analyzed by JASPAR. (B) Schematic of the strategy to screen the target genes of GATA5. (C) ChIP-qPCR showing the enrichment of GATA5 binding in the promoter of the putative target genes in HT29 cells with ectopic GATA5 expression. Data are shown as the mean ± SD of 3 independent repeated experiments. P value was calculated by 2-way ANOVA (*P < 0.05, **P < 0.01). (D) Luciferase reporter assay of the putative GATA5 target genes. HEK293T cells were transfected with luciferase vector containing promoter sequence of the putative GATA5 target genes together with GATA5 plasmids or empty vector, followed by luciferase activity measurement. Data are shown as the mean ± SD of 3 independent repeated experiments. P value was calculated by 2-way ANOVA (**P < 0.01, ***P < 0.001). (E) Western blot showing the expression levels of FGFR1, CLU, and WWTR1 in HT29 cells with ectopic expression of C6orf223 or C6orf223 together with GATA5. (F and G) Quantification (F) and representative IHC image (G) showing the expression level of C6orf223, GATA5, WWTR1, FGFR1, and CLU in primary cecum tumors from mice orthotopically injected with HT29 cells. Scale bar: 100 μm. Data are shown as the mean ± SEM of 5 mice per group. P value was calculated by 2-way ANOVA (NS; *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following open publication (https://www.jci.org/articles/view/186052), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of GATA-5 by Western Blot

Identification of GATA5 target genes in response to C6orf223-mediated CRC growth and metastasis.(A) Binding motif of GATA5 analyzed by JASPAR. (B) Schematic of the strategy to screen the target genes of GATA5. (C) ChIP-qPCR showing the enrichment of GATA5 binding in the promoter of the putative target genes in HT29 cells with ectopic GATA5 expression. Data are shown as the mean ± SD of 3 independent repeated experiments. P value was calculated by 2-way ANOVA (*P < 0.05, **P < 0.01). (D) Luciferase reporter assay of the putative GATA5 target genes. HEK293T cells were transfected with luciferase vector containing promoter sequence of the putative GATA5 target genes together with GATA5 plasmids or empty vector, followed by luciferase activity measurement. Data are shown as the mean ± SD of 3 independent repeated experiments. P value was calculated by 2-way ANOVA (**P < 0.01, ***P < 0.001). (E) Western blot showing the expression levels of FGFR1, CLU, and WWTR1 in HT29 cells with ectopic expression of C6orf223 or C6orf223 together with GATA5. (F and G) Quantification (F) and representative IHC image (G) showing the expression level of C6orf223, GATA5, WWTR1, FGFR1, and CLU in primary cecum tumors from mice orthotopically injected with HT29 cells. Scale bar: 100 μm. Data are shown as the mean ± SEM of 5 mice per group. P value was calculated by 2-way ANOVA (NS; *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following open publication (https://www.jci.org/articles/view/186052), licensed under a CC-BY license. Not internally tested by R&D Systems.