Human GIPR Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB8210R-100
Detection  of GIPR in HEK293 Human Cell Line Transfected with Human GIPR and eGFP by  Flow Cytometry.
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Human GIPR Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human GIPR in direct ELISAs. Stains human GIPR transfected cells but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG1 Clone # 591853
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GIPR
Met1-Cys466
Accession # P48546
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of GIPR in HEK293 Human Cell Line Transfected with Human GIPR and eGFP by  Flow Cytometry. View Larger

Detection of GIPR in HEK293 Human Cell Line Transfected with Human GIPR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human GIPR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human GIPR Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8210R). Quadrant markers were set based on control antibody staining (Catalog # IC002R). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GIPR

GIPR is a 7-transmembrane receptor for GIP (glucose-dependent insulinotropic polypeptide or gastric inhibitory polypeptide). The 466 amino acid (aa) human GIPR contains 176 extracellular domain (ECD) aa that share 77% and 81% aa identity with mouse and rat GIPR ECD, respectively. A splice isoform of 430 aa has a deletion of aa 58-93 in the N-terminal ECD, while isoforms of 491 and 419 aa have alternate C-terminal cytoplasmic sequences. Engagement by GIP on pancreatic b‑cells activates adenylate cyclase to regulate insulin compensation in the presence of high circulating glucose. GIPR is also expressed on adipocytes, osteoblasts and myelinating Schwann cell membranes.

Long Name
Gastric Inhibitory Polypeptide Receptor
Entrez Gene IDs
2696 (Human); 381853 (Mouse); 25024 (Rat)
Alternate Names
gastric inhibitory polypeptide receptor; GIPR; GIP-R; Glucose-dependent insulinotropic polypeptide receptor; MGC126722; PGQTL2

Product Datasheets

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