Detection of GIPR in HEK293 Human Cell Line Transfected with Human GIPR and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human GIPR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human GIPR Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8210R). Quadrant markers were set based on control antibody staining (Catalog # IC002R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
GIPR is a 7-transmembrane receptor for GIP (glucose-dependent insulinotropic polypeptide or gastric inhibitory polypeptide). The 466 amino acid (aa) human GIPR contains 176 extracellular domain (ECD) aa that share 77% and 81% aa identity with mouse and rat GIPR ECD, respectively. A splice isoform of 430 aa has a deletion of aa 58-93 in the N-terminal ECD, while isoforms of 491 and 419 aa have alternate C-terminal cytoplasmic sequences. Engagement by GIP on pancreatic b‑cells activates adenylate cyclase to regulate insulin compensation in the presence of high circulating glucose. GIPR is also expressed on adipocytes, osteoblasts and myelinating Schwann cell membranes.
Have you used Human GIPR Alexa Fluor® 647-conjugated Antibody?
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