Human GIPR Antibody

Catalog # Availability Size / Price Qty
MAB8210
MAB8210-SP
Detection of GIPR in HEK293 Human Cell Line Transfected with Human GIPR and eGFP by Flow Cytometry.
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human GIPR Antibody Summary

Species Reactivity
Human
Specificity
Detects human GIPR in direct ELISAs. Stains human GIPR transfected cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG1 Clone # 591853
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GIPR.
Met1-Cys466
Accession # P48546
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
Blockade of Receptor-ligand Interaction
At 2.5 ug/mL, this antibody will block >95% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of GIPR antibody in HEK293 Human Cell Line Transfected with Human GIPR and eGFP antibody by Flow Cytometry. View Larger

Detection of GIPR in HEK293 Human Cell Line Transfected with Human GIPR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human GIPR or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry GIPR antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

GIPR in Human Pancreas. GIPR was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Blockade of Receptor-ligand Interaction Human GIP binding to human GIPR-transfected HEK293 cells blocked by Human GIPR Antibody. View Larger

Human GIP binding to human GIPR-transfected HEK293 cells blocked by Human GIPR Antibody. In a functional flow cytometry test, biotinylated recombinant Human GIP (Catalog # 2257, 25 ng/mL) binds to Human GIPR-transfected HEK293 cells (black dotted line). Binding is completely blocked (orange histogram) by 2.5 µg/mL of Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210). Mouse IgG1Isotype Control (Catalog # MAB002) at 2.5 µg/mL was used as a control (blue line). Cells were stained with Sterptavidin-APC (Catalog # F0050).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GIPR

GIPR is a 7-transmembrane receptor for GIP (glucose-dependent insulinotropic polypeptide or gastric inhibitory polypeptide). The 466 amino acid (aa) human GIPR contains 176 extracellular domain (ECD) aa that share 77% and 81% aa identity with mouse and rat GIPR ECD, respectively. A splice isoform of 430 aa has a deletion of aa 58-93 in the N-terminal ECD, while isoforms of 491 and 419 aa have alternate C-terminal cytoplasmic sequences. Engagement by GIP on pancreatic b‑cells activates adenylate cyclase to regulate insulin compensation in the presence of high circulating glucose. GIPR is also expressed on adipocytes, osteoblasts and myelinating Schwann cell membranes.

Long Name
Gastric Inhibitory Polypeptide Receptor
Entrez Gene IDs
2696 (Human); 381853 (Mouse); 25024 (Rat)
Alternate Names
gastric inhibitory polypeptide receptor; GIPR; GIP-R; Glucose-dependent insulinotropic polypeptide receptor; MGC126722; PGQTL2

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Citations for Human GIPR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. GIPR antagonist antibodies conjugated to GLP-1 peptide are bispecific molecules that decrease weight in obese mice and monkeys
    Authors: Shu-Chen Lu, Michelle Chen, Larissa Atangan, Elizabeth A. Killion, Renee Komorowski, Yuan Cheng et al.
    Cell Reports Medicine
  2. Chronic glucose-dependent insulinotropic polypeptide receptor (GIPR) agonism desensitizes adipocyte GIPR activity mimicking functional GIPR antagonism
    Authors: EA Killion, M Chen, JR Falsey, G Sivits, T Hager, L Atangan, J Helmering, J Lee, H Li, B Wu, Y Cheng, MM Véniant, DJ Lloyd
    Nat Commun, 2020-10-05;11(1):4981.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Human GIPR Antibody
By Anonymous on 04/10/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Neural progenitor cells Species: Human