Human GIPR Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human GIPR in direct ELISAs. Stains human GIPR transfected cells but not irrelevant transfectants.
  • Source
    Monoclonal Mouse IgG1 Clone # 591853
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human GIPR.
    Met1-Cys466
    Accession # P48546
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    At 2.5 ug/mL, this antibody will block >95% of the binding.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of GIPR in HEK293 Human Cell Line Transfected with Human GIPR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human GIPR or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
Immunohistochemistry
GIPR in Human Pancreas. GIPR was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Blockade of Receptor-ligand Interaction
HumanGIP binding to human GIPR-transfected HEK293 cells blocked by Human GIPRAntibody. In a functional flow cytometry test, biotinylatedrecombinant Human GIP (Catalog # 2257,25 ng/mL) binds to Human GIPR-transfected HEK293 cells (black dotted line).Binding is completely blocked (orange histogram) by 2.5 μg/mL of MouseAnti-Human GIPR Monoclonal Antibody (Catalog # MAB8210). Mouse IgG1 IsotypeControl (Catalog # MAB002)at 2.5 μg/mL was used as a control (blue line). Cells were stained withSterptavidin-APC (Catalog # F0050).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GIPR
GIPR is a 7-transmembrane receptor for GIP (glucose-dependent insulinotropic polypeptide or gastric inhibitory polypeptide). The 466 amino acid (aa) human GIPR contains 176 extracellular domain (ECD) aa that share 77% and 81% aa identity with mouse and rat GIPR ECD, respectively. A splice isoform of 430 aa has a deletion of aa 58-93 in the N-terminal ECD, while isoforms of 491 and 419 aa have alternate C-terminal cytoplasmic sequences. Engagement by GIP on pancreatic b‑cells activates adenylate cyclase to regulate insulin compensation in the presence of high circulating glucose. GIPR is also expressed on adipocytes, osteoblasts and myelinating Schwann cell membranes.
  • Long Name:
    Gastric Inhibitory Polypeptide Receptor
  • Entrez Gene IDs:
    2696 (Human); 381853 (Mouse); 25024 (Rat)
  • Alternate Names:
    gastric inhibitory polypeptide receptor; GIPR; GIP-R; Glucose-dependent insulinotropic polypeptide receptor; MGC126722; PGQTL2
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Isotype Controls
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Mouse IgG1 Isotype Control

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Staining Reagents
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Flow Cytometry Mouse Lyse Buffer (10X)

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