Human GIPR Antibody Summary
Accession # P48546
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of GIPR in HEK293 Human Cell Line Transfected with Human GIPR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human GIPR or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
GIPR in Human Pancreas. GIPR was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Human GIP binding to human GIPR-transfected HEK293 cells blocked by Human GIPR Antibody. In a functional flow cytometry test, biotinylated recombinant Human GIP (Catalog # 2257, 25 ng/mL) binds to Human GIPR-transfected HEK293 cells (black dotted line). Binding is completely blocked (orange histogram) by 2.5 µg/mL of Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210). Mouse IgG1Isotype Control (Catalog # MAB002) at 2.5 µg/mL was used as a control (blue line). Cells were stained with Sterptavidin-APC (Catalog # F0050).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
GIPR is a 7-transmembrane receptor for GIP (glucose-dependent insulinotropic polypeptide or gastric inhibitory polypeptide). The 466 amino acid (aa) human GIPR contains 176 extracellular domain (ECD) aa that share 77% and 81% aa identity with mouse and rat GIPR ECD, respectively. A splice isoform of 430 aa has a deletion of aa 58-93 in the N-terminal ECD, while isoforms of 491 and 419 aa have alternate C-terminal cytoplasmic sequences. Engagement by GIP on pancreatic b‑cells activates adenylate cyclase to regulate insulin compensation in the presence of high circulating glucose. GIPR is also expressed on adipocytes, osteoblasts and myelinating Schwann cell membranes.
Citation for Human GIPR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Chronic glucose-dependent insulinotropic polypeptide receptor (GIPR) agonism desensitizes adipocyte GIPR activity mimicking functional GIPR antagonism
Authors: EA Killion, M Chen, JR Falsey, G Sivits, T Hager, L Atangan, J Helmering, J Lee, H Li, B Wu, Y Cheng, MM Véniant, DJ Lloyd
Nat Commun, 2020;11(1):4981.
Sample Types: Whole Cells
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