Human GIPR PE-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human GIPR in direct ELISAs. Stains human GIPR transfected cells but not irrelevant transfectants in flow cytometry.
  • Source
    Monoclonal Mouse IgG1 Clone # 591853
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human GIPR.
    Met1-Cys466
    Accession # P48546
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of GIPR in HEK293 Human Cell Line Transfected with Human GIPR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human GIPR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human GIPR PE‑conjugated Monoclonal Antibody (Catalog # FAB8210P). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: GIPR
GIPR is a 7-transmembrane receptor for GIP (glucose-dependent insulinotropic polypeptide or gastric inhibitory polypeptide). The 466 amino acid (aa) human GIPR contains 176 extracellular domain (ECD) aa that share 77% and 81% aa identity with mouse and rat GIPR ECD, respectively. A splice isoform of 430 aa has a deletion of aa 58-93 in the N-terminal ECD, while isoforms of 491 and 419 aa have alternate C-terminal cytoplasmic sequences. Engagement by GIP on pancreatic b‑cells activates adenylate cyclase to regulate insulin compensation in the presence of high circulating glucose. GIPR is also expressed on adipocytes, osteoblasts and myelinating Schwann cell membranes.
  • Long Name:
    Gastric Inhibitory Polypeptide Receptor
  • Entrez Gene IDs:
    2696 (Human); 381853 (Mouse); 25024 (Rat)
  • Alternate Names:
    gastric inhibitory polypeptide receptor; GIPR; GIP-R; Glucose-dependent insulinotropic polypeptide receptor; MGC126722; PGQTL2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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