Human Girdin Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Girdin in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Girdin
    Asn1510-Leu1824
    Accession # Q3V6T2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Girdin by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Girdin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5345) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Girdin at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Girdin
Girdin (GIRDers of actIN filaments; also APE and GIV) is a member of the CCDC88 family of molecules. It is a cytosolic protein, ubiquitously expressed, and plays a key role in the motility of breast cancer cells. Human Girdin is 1871 amino acids (aa) in length. It contains two coiled-coil regions that mediate homodimerization (aa 196-425 and 458-1358), and one phosphoinositide-binding domain (aa 1390-1408). Normally, Girdin is membrane-associated via an interaction with phosphoinositides. Upon phosphorylation at Ser1417 by Akt, it relocates to lamellipodia where it crosslinks actin filaments. Multiple isoforms of Girdin have been detected.
  • Long Name:
    Girders of Actin Filament
  • Entrez Gene IDs:
    55704 (Human); 108686 (Mouse); 305605 (Rat)
  • Alternate Names:
    Akt phosphorylation enhancer; APE; APEGIRDIN; CCDC88A; coiled-coil domain containing 88A; Coiled-coil domain-containing protein 88A; G alpha-interacting vesicle-associated protein; G{alpha}-interacting vesicle-associated protein; Girders of actin filament; Girdin; GIV; GIVFLJ10392; GRDN; GRDNAKT-phosphorylation enhancer; HkRP1; HkRP1Galpha-interacting vesicle-associated protein; Hook-related protein 1; KIAA1212DKFZp686D0630
Related Research Areas
Isotype Controls
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Normal Sheep IgG Biotinylated Control

Ctrl BAF020 1
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