Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Girdin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Girdin
Asn1510-Leu1824
Accession # Q3V6T2

Specificity

Detects endogenous human Girdin in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Girdin Antibody

Detection of Human Girdin antibody by Western Blot.

Detection of Human Girdin by Western Blot.

Western blot shows lysates of H69AR human small cell lung cancer cell line, MRC-5 human embryonic lung fibroblast cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Girdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5345) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Girdin at approximately 200 to 220 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Detection of Human Girdin by Simple WesternTM.

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Girdin at approximately 206 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Girdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5345) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.

Applications for Human Girdin Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: A431 human epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: H69AR human small cell lung cancer cell line, MRC-5 human embryonic lung fibroblast cell line, and A431 human epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Girdin

Girdin (GIRDers of actIN filaments; also APE and GIV) is a member of the CCDC88 family of molecules. It is a cytosolic protein, ubiquitously expressed, and plays a key role in the motility of breast cancer cells. Human Girdin is 1871 amino acids (aa) in length. It contains two coiled-coil regions that mediate homodimerization (aa 196-425 and 458-1358), and one phosphoinositide-binding domain (aa 1390-1408). Normally, Girdin is membrane-associated via an interaction with phosphoinositides. Upon phosphorylation at Ser1417 by Akt, it relocates to lamellipodia where it crosslinks actin filaments. Multiple isoforms of Girdin have been detected.

Long Name

Girders of Actin Filament

Alternate Names

APE, CCDC88A, GIV, GRDN, HkRP1

Entrez Gene IDs

55704 (Human); 108686 (Mouse); 305605 (Rat)

Gene Symbol

CCDC88A

UniProt

Q3V6T2

Additional Girdin Products

Product Documents for Human Girdin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Girdin Antibody

For research use only

Citations for Human Girdin Antibody

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