Human Girdin Antibody
Human Girdin Antibody Summary
Asn1510-Leu1824
Accession # Q3V6T2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Girdin by Western Blot. Western blot shows lysates of H69AR human small cell lung cancer cell line, MRC-5 human embryonic lung fibroblast cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Girdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5345) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Girdin at approximately 200 to 220 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
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Detection of Human Girdin by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Girdin at approximately 206 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Girdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5345) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Girdin
Girdin (GIRDers of actIN filaments; also APE and GIV) is a member of the CCDC88 family of molecules. It is a cytosolic protein, ubiquitously expressed, and plays a key role in the motility of breast cancer cells. Human Girdin is 1871 amino acids (aa) in length. It contains two coiled-coil regions that mediate homodimerization (aa 196-425 and 458-1358), and one phosphoinositide-binding domain (aa 1390-1408). Normally, Girdin is membrane-associated via an interaction with phosphoinositides. Upon phosphorylation at Ser1417 by Akt, it relocates to lamellipodia where it crosslinks actin filaments. Multiple isoforms of Girdin have been detected.
Product Datasheets
Citations for Human Girdin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Akt-Girdin signaling in cancer-associated fibroblasts contributes to tumor progression.
Authors: Yamamura Y, Asai N, Enomoto A, Kato T, Mii S, Kondo Y, Ushida K, Niimi K, Tsunoda N, Nagino M, Ichihara S, Furukawa K, Maeda K, Murohara T, Takahashi M
Cancer Res, 2015;75(5):813-23.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Loss of Dlg5 expression promotes the migration and invasion of prostate cancer cells via Girdin phosphorylation.
Authors: Tomiyama, L, Sezaki, T, Matsuo, M, Ueda, K, Kioka, N
Oncogene, 2015;34(9):1141-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Speed control for neuronal migration in the postnatal brain by Gmip-mediated local inactivation of RhoA.
Authors: Ota, Haruko, Hikita, Takao, Sawada, Masato, Nishioka, Tomoki, Matsumoto, Mami, Komura, Masayuki, Ohno, Akihisa, Kamiya, Yukiyo, Miyamoto, Takuya, Asai, Naoya, Enomoto, Atsushi, Takahashi, Masahide, Kaibuchi, Kozo, Sobue, Kazuya, Sawamoto, Kazunobu
Nat Commun, 2014;5(0):4532.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Girdin and its phosphorylation dynamically regulate neonatal vascular development and pathological neovascularization in the retina.
Authors: Ito T, Komeima K, Yasuma T, Enomoto A, Asai N, Asai M, Iwase S, Takahashi M, Terasaki H
Am J Pathol, 2013;182(2):586-96.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Proteomic analysis of Girdin-interacting proteins in migrating new neurons in the postnatal mouse brain.
Authors: Ota H, Hikita T, Nishioka T, Matsumoto M, Ito J, Asai N, Enomoto A, Takahashi M, Kaibuchi K, Sobue K, Sawamoto K
Biochem Biophys Res Commun, 2013;442(1):16-21.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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