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Human GLI-1 Alexa Fluor® 700-conjugated Antibody

Catalog #: AF3324N Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3324N-100UG

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Human GLI-1 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human GLI-1 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human GLI-3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GLI-1
Met1-Glu234
Accession # P08151
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 
Chromatin Immunoprecipitation (ChIP)
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GLI-1

GLI-1 is a transcription regulator with 5 conserved tandem C2-H2 zinc finger domains, joined by conserved histidine/cysteine linkers. It is a known oncogene that was originally isolated in a human glioblastoma. GLI-1 acts downstream of Hedgehog signaling and is involved in cell proliferation and pattern formation during embryonic development. Within the region used as the immunogen, human GLI-1 shares 81% amino acid sequence homology with mouse GLI-1.

Long Name
Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]
Entrez Gene IDs
2735 (Human); 14632 (Mouse); 140589 (Rat)
Alternate Names
GLI family zinc finger 1; GLI1; GLI-1; glioma-associated oncogene family zinc finger 1; glioma-associated oncogene homolog 1 (zinc finger protein); Glioma-associated oncogene; GLIzinc finger protein GLI1; Oncogene GLI

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