Human GLI-1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human GLI‑1 by Western Blot.
3 Images
Product Details
Citations (4)
Supplemental Products

Human GLI-1 Antibody Summary

Species Reactivity
Detects human GLI-1 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human GLI-3 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human GLI-1
Accession # P08151
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human GLI-1 antibody by Western Blot. View Larger

Detection of Human GLI‑1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human GLI-1. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3324) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GLI-1 at approximately 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chromatin Immunoprecipitation (ChIP) Detection of GLI-1-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of GLI‑1-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 mintes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-1/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3324) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. TheBcl-2promoter was detected by standard PCR.

Immunohistochemistry GLI-1 antibody in Human Skin by Immunohistochemistry (IHC-P). View Larger

GLI-1 in Human Skin. GLI-1 was detected in immersion fixed paraffin-embedded sections of human skin using Human GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3324 ) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLI-1

GLI-1 is a transcription regulator with 5 conserved tandem C2-H2 zinc finger domains, joined by conserved histidine/cysteine linkers. It is a known oncogene that was originally isolated in a human glioblastoma. GLI-1 acts downstream of Hedgehog signaling and is involved in cell proliferation and pattern formation during embryonic development. Within the region used as the immunogen, human GLI-1 shares 81% amino acid sequence homology with mouse GLI-1.

Long Name
Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]
Entrez Gene IDs
2735 (Human); 14632 (Mouse); 140589 (Rat)
Alternate Names
GLI family zinc finger 1; GLI1; GLI-1; glioma-associated oncogene family zinc finger 1; glioma-associated oncogene homolog 1 (zinc finger protein); Glioma-associated oncogene; GLIzinc finger protein GLI1; Oncogene GLI

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Citations for Human GLI-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. RNA-seq and ChIP-seq Identification of Unique and Overlapping Targets of GLI Transcription Factors in Melanoma Cell Lines
    Authors: M Kurtovi?, N Piteša, N Bartoni?ek, P Ozreti?, V Musani, J ?onkaš, T Petri?, C King, M Sabol
    Cancers, 2022-09-19;14(18):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  2. Two Cases of Temporomandibular Synovial Chondromatosis Associated with Gli1 Gene Mutation
    Authors: T Fukutani, S Toratani, T Kanda, K Matsui, S Yamasaki, K Sumi, I Ogawa, S Yanamoto
    International Journal of Environmental Research and Public Health, 2022-04-13;19(8):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction
    Authors: D Hasegawa, H Ochiai-Shi, S Onodera, T Nakamura, A Saito, T Onda, K Watanabe, K Nishimura, M Ohtaka, M Nakanishi, K Kosaki, A Yamaguchi, T Shibahara, T Azuma
    PLoS ONE, 2017-10-31;12(10):e0186879.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Hedgehog and Notch signaling regulate self-renewal of undifferentiated pleomorphic sarcomas.
    Authors: Wang CY, Wei Q, Han I
    Cancer Res., 2012-01-09;72(4):1013-22.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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