Human Glut3 Alexa Fluor® 488-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Glut3. Recognizes human Glut3 expression on human Glut3-transfected NS0 cells, but not the NS0 control transfectants. No cross-reactivity was observed with transfectants expressing human Glut1 or human Glut2.
  • Source
    Monoclonal Mouse IgG2B Clone # 202017
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human Glut3
    Accession # P11169
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Glut3 in NS0 Mouse Cell Line Transfected with Human Glut3 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human Glut3 was stained with Alexa Fluor® 488‑conjugated Mouse Anti-Human Glut3 Monoclonal Antibody (Catalog # FAB1415G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Glut3
Glut3 belongs to the facilitative glucose transporter protein family that comprises 13 members, and is designated SLC2A3 (solute carier family 2, member 3). It is an integral membrane protein with 12 transmembrane domains. Glut3 is the glucose transporter responsible for maintaining an adequate glucose supply to neurons (1, 2). It is also expressed in placenta and articular chondrocytes (3, 4).
  • References:
    1. Vannuci, S.J. et al. (1997) Glia 21:2.
    2. Mueckler, M. et al. (1997) Biochem. Soc. Trans. 25:951.
    3. Illsley, N.P. (2000) Placenta 21:14.
    4. Mobasheri, A. (2002) Cell Biol. Int. 26:297.
  • Long Name:
    Glucose Transporter Type 3
  • Entrez Gene IDs:
    6515 (Human); 20527 (Mouse)
  • Alternate Names:
    FLJ90380; Glut3; GLUT-3; GLUT3Glucose transporter type 3, brain; SLC2A3; solute carrier family 2 (facilitated glucose transporter), member 3; solute carrier family 2, facilitated glucose transporter member 3
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC0041G 3  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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