Human Glut3 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1415G-100UG
Detection  of Glut3 in NS0 Mouse Cell Line Transfected with Human Glut3 by Flow  Cytometry.
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Human Glut3 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glut3. Recognizes human Glut3 expression on human Glut3-transfected NS0 cells, but not the NS0 control transfectants. No cross-reactivity was observed with transfectants expressing human Glut1 or human Glut2.
Source
Monoclonal Mouse IgG2B Clone # 202017
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Glut3
Accession # P11169
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of Glut3 in NS0 Mouse Cell Line Transfected with Human Glut3 by Flow  Cytometry. View Larger

Detection of Glut3 in NS0 Mouse Cell Line Transfected with Human Glut3 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human Glut3 was stained with Alexa Fluor® 488‑conjugated Mouse Anti-Human Glut3 Monoclonal Antibody (Catalog # FAB1415G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glut3

Glut3 belongs to the facilitative glucose transporter protein family that comprises 13 members, and is designated SLC2A3 (solute carier family 2, member 3). It is an integral membrane protein with 12 transmembrane domains. Glut3 is the glucose transporter responsible for maintaining an adequate glucose supply to neurons (1, 2). It is also expressed in placenta and articular chondrocytes (3, 4).

References
  1. Vannuci, S.J. et al. (1997) Glia 21:2.
  2. Mueckler, M. et al. (1997) Biochem. Soc. Trans. 25:951.
  3. Illsley, N.P. (2000) Placenta 21:14.
  4. Mobasheri, A. (2002) Cell Biol. Int. 26:297.
Long Name
Glucose Transporter Type 3
Entrez Gene IDs
6515 (Human); 20527 (Mouse)
Alternate Names
FLJ90380; Glut3; GLUT-3; GLUT3Glucose transporter type 3, brain; SLC2A3; solute carrier family 2 (facilitated glucose transporter), member 3; solute carrier family 2, facilitated glucose transporter member 3

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