Human Glut3 Antibody MAB1415: R&D Systems

Human Glut3 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Glut3. Recognizes human Glut3 expression on human Glut3-transfected NS0 cells, but not the NS0 control transfectants. No cross-reactivity was observed with transfectants expressing human Glut1 or human Glut2.
  • Source
    Monoclonal Mouse IgG2B Clone # 202017
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human Glut3
    Accession # P11169
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Glut3 in NS0 Mouse Cell Line Transfected with Human Glut3 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human Glut3 was stained with Mouse Anti-Human Glut3 Monoclonal Antibody (Catalog # MAB1415, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
Glut3 in SH‑SY5Y Human Cell Line. Glut3 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Mouse Anti-Human Glut3 Monoclonal Antibody (Catalog # MAB1415) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glut3
Glut3 belongs to the facilitative glucose transporter protein family that comprises 13 members, and is designated SLC2A3 (solute carier family 2, member 3). It is an integral membrane protein with 12 transmembrane domains. Glut3 is the glucose transporter responsible for maintaining an adequate glucose supply to neurons (1, 2). It is also expressed in placenta and articular chondrocytes (3, 4).
  • References:
    1. Vannuci, S.J. et al. (1997) Glia 21:2.
    2. Mueckler, M. et al. (1997) Biochem. Soc. Trans. 25:951.
    3. Illsley, N.P. (2000) Placenta 21:14.
    4. Mobasheri, A. (2002) Cell Biol. Int. 26:297.
  • Long Name:
    Glucose Transporter Type 3
  • Entrez Gene IDs:
    6515 (Human); 20527 (Mouse)
  • Alternate Names:
    FLJ90380; Glut3; GLUT-3; GLUT3Glucose transporter type 3, brain; SLC2A3; solute carrier family 2 (facilitated glucose transporter), member 3; solute carrier family 2, facilitated glucose transporter member 3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. IGF-I increases the recruitment of GLUT4 and GLUT3 glucose transporters on cell surface in hyperthyroidism.
    Authors: Dimitriadis G, Maratou E, Boutati E
    Eur. J. Endocrinol., 2008;158(3):361-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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