Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Flow Cytometry, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 202017
View all Glut3 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Glut3
Accession # P11169

Specificity

Detects human Glut3. Recognizes human Glut3 expression on human Glut3-transfected NS0 cells, but not the NS0 control transfectants. No cross-reactivity was observed with transfectants expressing human Glut1 or human Glut2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Glut3 Antibody

Detection of Glut3 antibody in NS0 Mouse Cell Line Transfected with Human Glut3 antibody by Flow Cytometry.

Detection of Glut3 in NS0 Mouse Cell Line Transfected with Human Glut3 by Flow Cytometry.

NS0 mouse myeloma cell line transfected with human Glut3 was stained with Mouse Anti-Human Glut3 Monoclonal Antibody (Catalog # MAB1415, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Glut3 antibody in SH-SY5Y Human Cell Line by Immunocytochemistry (ICC).

Glut3 in SH‑SY5Y Human Cell Line.

Glut3 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human Glut3 Monoclonal Antibody (Catalog # MAB1415) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Glut3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: NS0 mouse myeloma cell line transfected with human Glut3

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line and immersion fixed Caco-2 human colorectal adenocarcinoma cell line

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Glut3

Glut3 belongs to the facilitative glucose transporter protein family that comprises 13 members, and is designated SLC2A3 (solute carier family 2, member 3). It is an integral membrane protein with 12 transmembrane domains. Glut3 is the glucose transporter responsible for maintaining an adequate glucose supply to neurons (1, 2). It is also expressed in placenta and articular chondrocytes (3, 4).

References

  1. Vannuci, S.J. et al. (1997) Glia 21:2.
  2. Mueckler, M. et al. (1997) Biochem. Soc. Trans. 25:951.
  3. Illsley, N.P. (2000) Placenta 21:14.
  4. Mobasheri, A. (2002) Cell Biol. Int. 26:297.

Long Name

Glucose Transporter Type 3

Alternate Names

SLC2A3

Entrez Gene IDs

6515 (Human); 20527 (Mouse)

Gene Symbol

SLC2A3

UniProt

P11169

Additional Glut3 Products

Product Documents for Human Glut3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Glut3 Antibody

For research use only

Citations for Human Glut3 Antibody

Customer Reviews for Human Glut3 Antibody

There are currently no reviews for this product. Be the first to review Human Glut3 Antibody and earn rewards!

Have you used Human Glut3 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies