Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2B Clone # 202017
View all Glut3 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Glut3
Met1-Val496
Accession # P11169

Specificity

Detects human Glut3. Stains human Glut3-transfected NS0 and CHO cells, but not control transfectants. In flow cytometry, no cross‑reactivity with human Glut1 or Glut2 transfectants is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Glut3 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Human Glut3 transfected NS0 cells

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Caco-2 human colorectal adenocarcinoma cell line

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glut3

Glut3 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut3 is the glucose transporter responsible for maintaining an adequate glucose supply to neurons (1, 2). It is also expressed in placenta and articular chondrocytes (3, 4).

References

  1. Vannuci, S.J. et al. (1997) Glia 21:2.
  2. Mueckler, M. et al. (1997) Biochem. Soc. Trans. 25:951.
  3. Illsley, N.P. (2000) Placenta 21:14.
  4. Mobasheri, A. (2002) Cell Biol. Int. 26:297.

Long Name

Glucose Transporter Type 3

Alternate Names

SLC2A3

Entrez Gene IDs

6515 (Human); 20527 (Mouse)

Gene Symbol

SLC2A3

UniProt

P11169

Additional Glut3 Products

Product Documents for Human Glut3 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Glut3 Biotinylated Antibody

For research use only

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Protocols

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