Human Glut4 Alexa Fluor® 647-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Glut4 in direct ELISAs.  Stains human Glut4 transfectants but not irrelevant transfectants in flow cytometry.
  • Source
    Monoclonal Mouse IgG2B Clone # 925932
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Glut4
    Met1-Asp509
    Accession # P14672
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Glut4 in HEK293 Human Cell Line Transfected with Human Glut4 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Glut4 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Glut4 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB86541R). Quadrant markers were set based on control antibody staining (Catalog # IC0041R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Glut4
Glut4 (Glucose Transporter Member 4), also known as SLC2A4, is a 509 aa multi-pass type membrane protein and shares 65% aa identity with mouse Glut4.  It is an insulin-regulated glucose transporter.  Glut4 is most highly expressed in adipose and striated muscle tissues, but has also been reported in multiple other tissues including the nervous system and breast cancer.  Insulin stimulated transport of Glut4 has been shown to be impaired in type 2 diabetes patients.  Additionally, inhibition of Glut4 may be useful in reducing proliferation of breast cancer cells.
  • Entrez Gene IDs:
    6517 (Human); 20528 (Mouse); 25139 (Rat)
  • Alternate Names:
    Glut4; GLUT-4; GLUT4Glucose transporter type 4, insulin-responsive; insulin-responsive glucose transporter type 4; SLC2A4; solute carrier family 2 (facilitated glucose transporter), member 4; solute carrier family 2, facilitated glucose transporter member 4
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 647-conjugated Isotype Control

Ctrl IC0041R
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 4
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 2
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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