Detection of Glut4 in HEK293 Human Cell Line Transfected with Human Glut4 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human Glut4 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Glut4 Monoclonal Antibody (Catalog # MAB86541) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Glut4 (Glucose Transporter Member 4), also known as SLC2A4, is a 509 aa multi-pass type membrane protein and shares 65% aa identity with mouse Glut4. It is an insulin-regulated glucose transporter. Glut4 is most highly expressed in adipose and striated muscle tissues, but has also been reported in multiple other tissues including the nervous system and breast cancer. Insulin stimulated transport of Glut4 has been shown to be impaired in type 2 diabetes patients. Additionally, inhibition of Glut4 may be useful in reducing proliferation of breast cancer cells.
Entrez Gene IDs:
6517 (Human); 20528 (Mouse); 25139 (Rat)
Glut4; GLUT-4; GLUT4Glucose transporter type 4, insulin-responsive; insulin-responsive glucose transporter type 4; SLC2A4; solute carrier family 2 (facilitated glucose transporter), member 4; solute carrier family 2, facilitated glucose transporter member 4
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