Human Glypican 3 Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Glypican 3 in ELISAs and Western blots. Does not cross-react with recombinant human (rh) Glypican 2, rhGlypican 5, or rhGlypican 6.
  • Source
    Monoclonal Mouse IgG2A Clone # 307801
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Glypican 3
    Accession # P51654.1
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Glypican 3 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glypican 3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB2119G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Glypican 3

Glypicans (GPC) are a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1-GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. While glypican proteins do not share strong amino acid sequence identity (they range from 17-63%), the conserved cysteine residues in their CRDs suggests similarity in their three‑dimensional structure (1, 2).

Mutations in GPC3 cause a rare disorder in humans, Simpson-Golabi-Behmel Syndrome, which is characterized by pre and postnatal overgrowth of multiple tissues and organs and an increased risk for developing embryonic tumors (3). These features are also present in the mouse knock-out of GPC3 indicating that GPC3 regulates cell survival and inhibits cell proliferation during development (4). Glypican 3 has been implicated in regulating many different signaling pathways including: IGF, FGF, BMP, and Wnt. An endoproteolytic processing of GPC3 by proprotein convertases is required for the modulation of Wnt signaling (5). Direct interaction with FGF-basic has been observed and is mediated by the heparan sulfate chains (6).

  • References:
    1. Filmus, J. and S.B. Selleck (2001) J. Clinical Invest. 108:497. 
    2. De Cat, B and G. David (2001) Seminars in Cell & Dev. Biol. 12:117. 
    3. Pilia, G. et al. (1996) Nat. Genet. 12: 241. 
    4. Cano-Gauci, D.F. et al. (1999) J. Cell Biol. 146: 255.
    5. De Cat, B. et al. (2003) J. Cell Biol. 163:625.
    6. Song, H.H. et al. (1997) J. Biol. Chem. 272:7574.
  • Entrez Gene IDs:
    2719 (Human); 14734 (Mouse); 25236 (Rat)
  • Alternate Names:
    DGSX; glypican 3; glypican proteoglycan 3; glypican-3; GPC3; GTR2-2; heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; OCI5; OCI-5; secreted glypican-3; SGB; SGBS; SGBS1SDYS
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Alexa Fluor® 488-conjugated Antibody

Ctrl IC003G  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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