Human Glypican 6 Alexa Fluor® 405-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Glypican 6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Glypican 2, 3, or 5 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 348701
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Glypican 6
    Asp24-Val527
    Accession # Q9Y625
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 405
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Glypican 6 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Alexa Fluor® 405‑conjugated Mouse Anti-Human Glypican 6 Monoclonal Antibody (Catalog # FAB2845V, filled histogram) or isotype control antibody (Catalog # IC002V, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Glypican 6

The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked heparan sulfate (HS) proteoglycans that likely play a key role in embryonic morphogenesis (1-4). There are currently six known mammalian Glypicans. They all share a common-sized protein core of 60‑70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C‑terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known, with one subfamily containing Glypicans 1, 2, 4 and 6, and another subfamily containing Glypicans 3 and 5 (3, 5). Human Glypican 6 (GPC-6) is synthesized as a 554 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 505 aa mature region and a 26 aa C-terminal prosegment (5, 6). There are four consecutive Ser-Gly repeats that serve as a heparin sulfate attachment site. GPC-6 is reported to be as large as 110 kDa in size. This translates into approximately 50 kDa of proteoglycan (5). Human to mouse, there is 97% aa identity over the entire GPC-6 molecule. Cells known to express GPC-6 are adult ovary and embryonic vascular and visceral smooth muscle, plus mesenchyme (embryonic connective tissue) in multiple organs (1, 5, 6). The function of GPC-6 is essentially unknown. As a Glypican family member, it may facilitate heparin-binding growth factor signaling and polyamine uptake into expressing cells (7, 8). In this regard, it would appear that GPC-6 with its attendant HS is down‑regulated by triiodothyronine during cartilage maturation, thus limiting the availability of sites for FGF sequestration and activity (9).

  • References:
    1. Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241. 
    2. Filmus, J. (2001) Glycobiology 11:19R. 
    3. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117. 
    4. Filmus, J. (2003) Glycoconj. J. 19:319. 
    5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
    6. Paine-Saunders, S. et al. (1999) Genomics 57:455.
    7. Fransson, L-A. et al. (2004) Cell Mol. Life Sci. 61:1016.
    8. Fransson, L-A. (2003) Int. J. Biochem. Cell Biol. 35:125.
    9. Bassett, J.H.D. et al. (2006) Endocrinology 147:295.
  • Entrez Gene IDs:
    10082 (Human); 23888 (Mouse)
  • Alternate Names:
    Glypican 6; glypican proteoglycan 6; glypican-6; GPC6; MGC126288; OMIMD1
Related Research Areas

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 405-conjugated Antibody

Ctrl IC002V
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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