Human Glypican 6 Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2845V-100UG
Detection  of Glypican 6 in HepG2 Human Cell Line by Flow Cytometry.
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Human Glypican 6 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glypican 6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Glypican 2, 3, or 5 is observed.
Source
Monoclonal Mouse IgG1 Clone # 348701
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Glypican 6
Asp24-Val527
Accession # Q9Y625
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of Glypican 6 in HepG2 Human Cell Line by Flow Cytometry. View Larger

Detection of Glypican 6 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Alexa Fluor® 405‑conjugated Mouse Anti-Human Glypican 6 Monoclonal Antibody (Catalog # FAB2845V, filled histogram) or isotype control antibody (Catalog # IC002V, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glypican 6

The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked heparan sulfate (HS) proteoglycans that likely play a key role in embryonic morphogenesis (1-4). There are currently six known mammalian Glypicans. They all share a common-sized protein core of 60‑70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C‑terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known, with one subfamily containing Glypicans 1, 2, 4 and 6, and another subfamily containing Glypicans 3 and 5 (3, 5). Human Glypican 6 (GPC-6) is synthesized as a 554 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 505 aa mature region and a 26 aa C-terminal prosegment (5, 6). There are four consecutive Ser-Gly repeats that serve as a heparin sulfate attachment site. GPC-6 is reported to be as large as 110 kDa in size. This translates into approximately 50 kDa of proteoglycan (5). Human to mouse, there is 97% aa identity over the entire GPC-6 molecule. Cells known to express GPC-6 are adult ovary and embryonic vascular and visceral smooth muscle, plus mesenchyme (embryonic connective tissue) in multiple organs (1, 5, 6). The function of GPC-6 is essentially unknown. As a Glypican family member, it may facilitate heparin-binding growth factor signaling and polyamine uptake into expressing cells (7, 8). In this regard, it would appear that GPC-6 with its attendant HS is down‑regulated by triiodothyronine during cartilage maturation, thus limiting the availability of sites for FGF sequestration and activity (9).

References
  1. Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241. 
  2. Filmus, J. (2001) Glycobiology 11:19R. 
  3. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117. 
  4. Filmus, J. (2003) Glycoconj. J. 19:319. 
  5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
  6. Paine-Saunders, S. et al. (1999) Genomics 57:455.
  7. Fransson, L-A. et al. (2004) Cell Mol. Life Sci. 61:1016.
  8. Fransson, L-A. (2003) Int. J. Biochem. Cell Biol. 35:125.
  9. Bassett, J.H.D. et al. (2006) Endocrinology 147:295.
Entrez Gene IDs
10082 (Human); 23888 (Mouse)
Alternate Names
Glypican 6; glypican proteoglycan 6; glypican-6; GPC6; MGC126288; OMIMD1

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