Human GM-CSF Antibody

(15 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human GM-CSF in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 3209
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human GM-CSF
    Ala18-Glu144
    Accession # P04141
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human GM-CSF (Catalog # 215-GM)
    under non-reducing conditions only
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize GM‑CSF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.075-0.45 µg/mL in the presence of 0.1 ng/mL Recombinant Human GM‑CSF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by GM‑CSF and Neutralization by Human GM‑CSF Antibody. Recombinant Human GM‑CSF (Catalog # 215-GM) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human GM‑CSF (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human GM‑CSF Monoclonal Antibody (Catalog # MAB215). The ND50 is typically 0.075-0.45 µg/mL.
Immunocytochemistry
GMCSF in Human PBMCs. Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was detected in human peripheral blood mononuclear cells (PBMCs) using Human GM-CSF Monoclonal Antibody (Catalog # MAB215) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GM-CSF

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a growth factor produced by a variety of lymphoid cells. It binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta chain that is shared by the high-affinity receptors for IL-3 and IL-5.

  • Long Name:
    Granulocyte Macrophage Growth Factor
  • Entrez Gene IDs:
    1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
  • Alternate Names:
    colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; molgramostin; sargramostim
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Epstein-Barr virus-induced VEGF and GM-CSF drive nasopharyngeal carcinoma metastasis via recruitment and activation of macrophages
    Authors: D Huang, S Song, ZZ Wu, W Wu, X Cui, JN Chen, MS Zeng, S Su
    Cancer Res., 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. GM-CSF promotes migration of human monocytes across the blood brain barrier.
    Authors: Vogel D, Kooij G, Heijnen P, Breur M, Peferoen L, van der Valk P, de Vries H, Amor S, Dijkstra C
    Eur J Immunol, 2015;45(6):1808-19.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  3. Sphingosine 1 Phosphate at the Blood Brain Barrier: Can the Modulation of S1P Receptor 1 Influence the Response of Endothelial Cells and Astrocytes to Inflammatory Stimuli?
    Authors: Spampinato S, Obermeier B, Cotleur A, Love A, Takeshita Y, Sano Y, Kanda T, Ransohoff R
    PLoS ONE, 2015;10(7):e0133392.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells.
    Authors: Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, Barra C, Comerma L, Chudnovskiy A, Gentile M, Llige D, Cols M, Serrano S, Arostegui J, Juan M, Yague J, Merad M, Fagarasan S, Cerutti A
    Nat Immunol, 2014;15(4):354-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis.
    Authors: Weber G, Chousterman B, Hilgendorf I, Robbins C, Theurl I, Gerhardt L, Iwamoto Y, Quach T, Ali M, Chen J, Rothstein T, Nahrendorf M, Weissleder R, Swirski F
    J Exp Med, 2014;211(6):1243-56.
  6. BCR-ABL-induced deregulation of the IL-33/ST2 pathway in CD34+ progenitors from chronic myeloid leukemia patients.
    Authors: Levescot A, Flamant S, Basbous S, Jacomet F, Feraud O, Anne Bourgeois E, Bonnet M, Giraud C, Roy L, Barra A, Chomel J, Turhan A, Guilhot F, Girard J, Gombert J, Herbelin A
    Cancer Res, 2014;74(10):2669-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  7. A positive feedback loop between mesenchymal-like cancer cells and macrophages is essential to breast cancer metastasis.
    Authors: Su S, Liu Q, Chen J, Chen J, Chen F, He C, Huang D, Wu W, Lin L, Huang W, Zhang J, Cui X, Zheng F, Li H, Yao H, Su F, Song E
    Cancer Cell, 2014;25(5):605-20.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  8. The implication of aberrant GM-CSF expression in decidual cells in the pathogenesis of preeclampsia.
    Authors: Huang SJ, Zenclussen AC, Chen CP, Basar M, Yang H, Arcuri F, Li M, Kocamaz E, Buchwalder L, Rahman M, Kayisli U, Schatz F, Toti P, Lockwood CJ
    Am. J. Pathol., 2010;177(5):2472-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  9. Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB.
    Authors: Choi W, Miyamura Y, Wolber R, Smuda C, Reinhold W, Liu H, Kolbe L, Hearing VJ
    J. Invest. Dermatol., 2010;130(6):1685-96.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  10. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007;117(12):3664-3672.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  11. Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
    Authors: Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD
    Arthritis Rheum., 2006;54(7):2096-108.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Immunodepletion
  12. Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
    Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
    Int. J. Cancer, 2006;119(10):2313-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  13. Thymus and activation-regulated chemokine (TARC/CCL17) produced by mouse epidermal Langerhans cells is upregulated by TNF-alpha and IL-4 and downregulated by IFN-gamma.
    Authors: Xiao T, Fujita H, Saeki H, Mitsui H, Sugaya M, Tada Y, Kakinuma T, Torii H, Nakamura K, Asahina A, Tamaki K
    Cytokine, 2003;23(4):126-32.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  14. Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects.
    Authors: Hellman C, Hallden G, Hylander B, Lundahl J
    Clin. Exp. Immunol., 2003;131(1):75-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  15. Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor.
    Authors: Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C
    Blood, 2002;99(1):224-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Expand to show all 15 Citations
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