Human GPR3 Antibody

MAB7429 has been discontinued. View all GPR3 products.
    
Datasheet / CoA / SDS
Product Details
FAQs
Reviews
  • Species Reactivity
    Human
  • Specificity
    Detects human GPR3 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 669867
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human GPR3
    Accession # NP_005272
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human GPR3 by Western Blot. Western blot shows lysates of human brain (Alzheimer's disease outer cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GPR3 Monoclonal Antibody (Catalog # MAB7429) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GPR3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of GPR3 in HEK293 Human Cell Line Transfected with human GRP3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human GRP3 or (B) irrelevant transfectants, and eGFP was stained with Mouse Anti-Human GPR3 Monoclonal Antibody (Catalog # MAB7429) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.
Immunohistochemistry
GPR3 in Human Brain. GPR3 was detected in immersion fixed paraffin-embedded sections of Alzheimer's human brain using Mouse Anti-Human GPR3 Monoclonal Antibody (Catalog # MAB7429) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPR3
GPR3, previously called ACCA (adenylate cyclase constitutive activator), is 7-transmembrane receptor with potential sites for glycosylation at the N-terminus and serine phosphorylation at the C-terminus. It has been shown to promote cAMP accumulation, which regulates maintenance of meiotic arrest in oocytes. It is also expressed in the brain, where it has been implicated in cAMP-dependent emotional responses and modulation of amyloid-beta peptide generation. The 330 amino acid (aa) human GPR3 contains 86 extracellular aa that share ~83% aa identity with mouse and rat GPR3.
  • Long Name:
    G Protein-coupled Receptor 3
  • Entrez Gene IDs:
    2827 (Human); 14748 (Mouse); 266769 (Rat)
  • Alternate Names:
    ACCA; G protein-coupled receptor 3; GPR3
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Have you used Human GPR3 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Contact Us to Order
reload

Feedback

Customer Information
Related Products
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies
R&D Systems Guarantee