Human Granzyme B Quantikine HS ELISA Kit

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HSGZB0
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Human Granzybe B HS ELISA
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Human Granzyme B Quantikine HS ELISA Kit Summary

Assay Length
4.0 Hours
Sample Type & Volume Required Per Well
Serum (50 uL), Plasma (50 uL), Heparin Plasma (13 uL), Saliva (25 uL)
Sensitivity
0.412 pg/mL
Assay Range
1.1 - 70 pg/mL (Serum, Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human Granzyme B.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® HS Human Granzyme B Immunoassay is a 4.0 hour solid-phase ELISA designed to measure human Granzyme B in serum, plasma and saliva. It contains NS0-expressed recombinant human Granzyme B and antibodies raised against the recombinant protein. Results obtained using natural human Granzyme B showed linear curves that were parallel to
the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Granzyme B.

Precision

Intra-Assay Precision (Precision within an assay) <div>Three samples of known concentration were tested twenty times on one plate to assess&nbsp;intra-assay precision.</div>
Inter-Assay Precision (Precision between assays) <div>Three samples of known concentration were tested in twenty separate assays to assess&nbsp;inter-assay precision. Assays were performed by at least three technicians.</div>

Serum, Plasma, Heparin Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 10.5 16.5 42.8 10.8 17 42.9
Standard Deviation 0.117 0.267 0.566 0.627 0.83 2.02
CV% 1.1 1.6 1.3 5.8 4.9 4.7

Recovery

The recovery of human Granzyme B spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 106 101-111
Heparin Plasma (n=4) 103 97-109
Saliva (n=4) 100 95-105
Serum (n=4) 108 101-115

Data Examples

Human Granzybe B HS ELISA

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Granzyme B

Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.

Entrez Gene IDs:
3002 (Human); 14939 (Mouse)
Alternate Names:
C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GRB; GrzB; GZMB; HLP; SECT

FAQs

No product specific FAQs exist for this product, however you may

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