HAI-1 is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matriptase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).
Human HAI‑1 Biotinylated Antibody
R&D Systems | Catalog # BAM10482
Loading...
Key Product Details
Species Reactivity
Human
Applications
ELISA Detection (Matched Antibody Pair)
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # 169428
Loading...
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HAI-1
Pro37-Val449
Accession # O43278
Pro37-Val449
Accession # O43278
Specificity
Detects human HAI-1 In ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant mouse HAI-1, recombinant human (rh) TFPI, rhTFPI-2, rhAPP, rhHAI-2A, rhGASP-1, or rhGASP-2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human HAI‑1 Biotinylated Antibody
Human HAI-1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: HAI-1
References
- Denda, K. et al. (2002) J. Biol. Chem. 277:14053.
- Shimomura, T. et al. (1997) J. Biol. Chem. 272:6370.
- Kataoka, H. et al. (2000) J. Biol. Chem. 275:40453.
Long Name
HGF Activator Inhibitor Type 1
Alternate Names
HAI1, SPINT1
Gene Symbol
SPINT1
UniProt
Additional HAI-1 Products
Product Documents for Human HAI‑1 Biotinylated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human HAI‑1 Biotinylated Antibody
For research use only
Related Research Areas
Customer Reviews for Human HAI‑1 Biotinylated Antibody
There are currently no reviews for this product. Be the first to review Human HAI‑1 Biotinylated Antibody and earn rewards!
Have you used Human HAI‑1 Biotinylated Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Loading...