Human HAI-1 Antibody

Catalog # Availability Size / Price Qty
MAB10481
MAB10481-SP
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Human HAI-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HAI-1 in ELISAs. In sandwich ELISAs, no cross-reactivity with recombinant mouse HAI-1, recombinant human (rh) TFPI, rhTFPI-2, rhAPP, rhHAI-2A, rhGASP-1, or rhGASP-2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 169409
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HAI-1
Pro37-Glu449
Accession # NP_003701
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample

Human HAI-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human HAI‑1 Biotinylated Antibody (Catalog # BAM10482)

Standard: Recombinant Human HAI-1 Protein, CF (Catalog # 1048-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-1

HAI-1 is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matriptase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

References
  1. Denda, K. et al. (2002) J. Biol. Chem. 277:14053. 
  2. Shimomura, T. et al. (1997) J. Biol. Chem. 272:6370.
  3. Kataoka, H. et al. (2000) J. Biol. Chem. 275:40453.
Long Name
HGF Activator Inhibitor Type 1
Entrez Gene IDs
6692 (Human); 20732 (Mouse)
Alternate Names
HAI; HAI1; HAI-1; hepatocyte growth factor activator inhibitor 1; Hepatocyte growth factor activator inhibitor type 1; kunitz-type protease inhibitor 1; MANSC2; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; SPINT1

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