Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 245106
View all HM74A/PUMA-G/GPR109A Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human HM74A/PUMA-G/GPR109A
Met1-Pro363
Accession # Q8TDS4

Specificity

Detects human HM74A/PUMA-G/GPR109A. Stains human HM74A/PUMA-G/GPR109A-transfected cells but not irrelevant transfectants. Cross-reactivity with HM74 (GPR109B) was not tested.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human HM74A/PUMA-G/GPR109A APC‑conjugated Antibody

Detection of HM74A/PUMA-G/GPR109A antibody in Human Blood Neutrophils antibody by Flow Cytometry.

Detection of HM74A/PUMA-G/GPR109A in Human Blood Neutrophils by Flow Cytometry.

Human peripheral blood neutrophils were stained with Rat Anti-Human HM74A/PUMA-G/GPR109A APC-conjugated Monoclonal Antibody (Catalog # FAB2760A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of HM74A/PUMA-G/GPR109A antibody in HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP antibody by Flow Cytometry.

Detection of HM74A/PUMA-G/GPR109A in HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human HM74A/PUMA-G/GPR109A or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Human HM74A/PUMA-G/GPR109A APC-conjugated Monoclonal Antibody (Catalog # FAB2760A). Quadrant markers were set based on control antibody staining (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human HM74A/PUMA-G/GPR109A APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood neutrophils and HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HM74A/PUMA-G/GPR109A

HM74A/GPR109A (also known as PUMA-G in mouse) is a 50-55 kDa 7-transmembrane protein member of a small subfamily within the GPCR #1 family. It is expressed on or in a wide variety of cells, including microglia, neutrophils, islet beta -cells, macrophages, adipocytes, retinal pigment epithelium and keratinocytes. Although HM74A is noted for its ability to bind niacin, it natural ligand is beta -hydroxybutyrate. Thus it may naturally act as a negative regulator of fatty acid release by fat, which is converted to ketone bodies by the liver, and which feedback on adipocytes. Full-length human HM74A shares 95% amino acid (aa) sequence identity with human HM74 and 81% aa sequence identity with mouse HM74A.

Long Name

G Protein-coupled Receptor HM74A/G Protein-coupled Receptor 109A

Alternate Names

GPR109A, HCA2, HCAR2, Niacr1, Nicotinic Acid Receptor, PUMAG

Entrez Gene IDs

338442 (Human); 80885 (Mouse)

Gene Symbol

HCAR2

UniProt

Q8TDS4

Additional HM74A/PUMA-G/GPR109A Products

Product Documents for Human HM74A/PUMA-G/GPR109A APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human HM74A/PUMA-G/GPR109A APC‑conjugated Antibody

For research use only

Related Research Areas

Obesity

Citations for Human HM74A/PUMA-G/GPR109A APC‑conjugated Antibody

Customer Reviews for Human HM74A/PUMA-G/GPR109A APC‑conjugated Antibody

There are currently no reviews for this product. Be the first to review Human HM74A/PUMA-G/GPR109A APC‑conjugated Antibody and earn rewards!

Have you used Human HM74A/PUMA-G/GPR109A APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies