Human HM74A/PUMA-G/GPR109A Fluorescein-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human HM74A/GPR109A. Stains human HM74A/GPR109A-transfected cells but not irrelevant transfectants. Cross-reactivity with HM74 (GPR109B) was not tested.
  • Source
    Monoclonal Rat IgG2B Clone # 245106
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human HM74A/GPR109A
    Accession # Q8TDS4
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of HM74A/PUMA-G/GPR109A in Human Neutrophils by Flow Cytometry. Human neutrophils were stained with Rat Anti-Human HM74A/PUMA-G/GPR109A Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB2760F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: HM74A/PUMA-G/GPR109A

HM74A/GPR109A (also known as PUMA-G in mouse) is a 50-55 kDa 7-transmembrane protein member of a small subfamily within the GPCR #1 family. It is expressed on or in a wide variety of cells, including microglia, neutrophils, islet beta -cells, macrophages, adipocytes, retinal pigment epithelium and keratinocytes. Although HM74A is noted for its ability to bind niacin, it natural ligand is beta -hydroxybutyrate. Thus it may naturally act as a negative regulator of fatty acid release by fat, which is converted to ketone bodies by the liver, and which feedback on adipocytes. Full-length human HM74A shares 95% amino acid (aa) sequence identity with human HM74 and 81% aa sequence identity with mouse HM74A.

  • Long Name:
    G Protein-coupled Receptor HM74A/G Protein-coupled Receptor 109A
  • Entrez Gene IDs:
    338442 (Human); 80885 (Mouse)
  • Alternate Names:
    GPR109A; HCA2; HCAR2; hydroxycarboxylic acid receptor 2; Niacr1; Nicotinic Acid Receptor; PUMAG
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Fluorescein-conjugated Antibody

Ctrl IC013F 1  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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