Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 245106
View all HM74A/PUMA-G/GPR109A Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human HM74A/GPR109A
Met1-Pro363
Accession # Q8TDS4

Specificity

Detects human HM74A/GPR109A. Stains human HM74A/GPR109A-transfected cells but not irrelevant transfectants. Cross-reactivity with HM74 (GPR109B) was not tested.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human HM74A/PUMA-G/GPR109A Antibody

Detection of HM74A/PUMA-G/GPR109A antibody in Human Blood Neutrophils antibody by Flow Cytometry.

Detection of HM74A/PUMA-G/GPR109A in Human Blood Neutrophils by Flow Cytometry.

Human peripheral blood neutrophils were stained with (A) Rat Anti-Human HM74A/PUMA-G/GPR109A Monoclonal Antibody (Catalog # MAB2760) or (B) isotype control antibody (Catalog # MAB0061) followed by anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113) and Mouse anti-Human Fc gamma RIII (CD16) PE-conjugated Monoclonal Antibody (Catalog # FAB2546P. View our protocol for Staining Membrane-associated Proteins.
Detection of HM74A/PUMA-G/GPR109A antibody in HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP antibody by Flow Cytometry.

Detection of HM74A/PUMA-G/GPR109A in HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human HM74A/PUMA-G/GPR109A or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Human HM74A/PUMA-G/GPR109A Monoclonal Antibody (Catalog # MAB2760) followed by anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB0061). View our protocol for Staining Membrane-associated Proteins.

Applications for Human HM74A/PUMA-G/GPR109A Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human whole blood monocytes and neutrophils and HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: HM74A/PUMA-G/GPR109A

HM74A, also known as GPR109A, is a high affinity G-protein coupled receptor for nicotinic acid. It is primarily expressed in adipose tissue and spleen.

Long Name

G Protein-coupled Receptor HM74A/G Protein-coupled Receptor 109A

Alternate Names

GPR109A, HCA2, HCAR2, Niacr1, Nicotinic Acid Receptor, PUMAG

Entrez Gene IDs

338442 (Human); 80885 (Mouse)

Gene Symbol

HCAR2

UniProt

Q8TDS4

Additional HM74A/PUMA-G/GPR109A Products

Product Documents for Human HM74A/PUMA-G/GPR109A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human HM74A/PUMA-G/GPR109A Antibody

For research use only

Related Research Areas

Obesity

Citations for Human HM74A/PUMA-G/GPR109A Antibody

Customer Reviews for Human HM74A/PUMA-G/GPR109A Antibody

There are currently no reviews for this product. Be the first to review Human HM74A/PUMA-G/GPR109A Antibody and earn rewards!

Have you used Human HM74A/PUMA-G/GPR109A Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies