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Human HSP47 Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB91662S Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB91662S-100UG

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Human HSP47 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human HSP47 in direct ELISAs and Western blots.  In Western blots, no cross-reactivity with mouse HSP47 is observed.
Source
Monoclonal Mouse IgG1 Clone # 950828
Purification
Protein A or G purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human HSP47
Ala19-Asp412
Accession # P50454
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: HSP47

Heat Shock Protein 47 (HSP47), also known as Serpin-H1/CBP1/CBP2, is localized to endoplasmic reticulum (ER), where it is a collagen-specific molecular chaperone.  In the ER, HSP47 interacts with and stabilizes correctly-folded procollagen. Nucleotide polymorphisms may be associated with preterm birth and Osteogenesis Imperfecta type X.  Serpin-H1 is up-regulated in several cancers including squamous cell carcinoma, breast and prostate carcinomas.  Expression in tumors drives malignant growth and invasion by enhancing deposition of extracellular matrix proteins.

Long Name
Heat Shock Protein 47
Entrez Gene IDs
871 (Human); 12406 (Mouse); 29345 (Rat)
Alternate Names
(collagen binding protein 1); Arsenic-transactivated protein 3; AsTP3; BERF-1; CBP1; CBP2; CBP2RA-A47; Cell proliferation-inducing gene 14 protein; Collagen-binding protein; colligen; Colligin; colligin-1; colligin-2; gp46; HSP47; HSP47serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47); member 1, (collagen binding protein 1); member 2; member 2, (collagen-binding protein 2); OI10; PPROM; proliferation-inducing gene 14; RA-A47; rheumatoid arthritis antigen A-47,47 kDa heat shock protein; Rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47); Serpin H1; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1; SERPINH1; SERPINH2colligin

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