Human HSP47 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human HSP47 in direct ELISAs and Western blots.  In Western blots, no cross-reactivity with mouse HSP47 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 950828
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human HSP47
    Ala19-Asp412
    Accession # P50454
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Simple Western
    1 µg/mL
    See below
  • Immunohistochemistry
    5-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human HSP47 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and JAR human choriocarcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91662) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HSP47 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
HSP47 in Human Breast Cancer Tissue. HSP47 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91662) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human HSP47 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP47 at approximately 57 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91662). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HSP47
Heat Shock Protein 47 (HSP47), also known as Serpin-H1/CBP1/CBP2, is localized to endoplasmic reticulum (ER), where it is a collagen-specific molecular chaperone.  In the ER, HSP47 interacts with and stabilizes correctly-folded procollagen. Nucleotide polymorphisms may be associated with preterm birth and Osteogenesis Imperfecta type X.  Serpin-H1 is up-regulated in several cancers including squamous cell carcinoma, breast and prostate carcinomas.  Expression in tumors drives malignant growth and invasion by enhancing deposition of extracellular matrix proteins.
  • References:
    1. Christiansen HE, et al, (2010) Am. J. Hum. Genet. 86:3892.
    2. Tasab M, et al, (2000) EMBO J. 19:22043.
    3. Kwon YJ, et al, (2009) Oncol Res. 18:1414.
    4. Zhu J, et al, (2015) Cancer Res. 75:15805.
    5. Nese N, et al, (2010) Anal Quant Cytol Histol. 32:90
  • Long Name:
    Heat Shock Protein 47
  • Entrez Gene IDs:
    871 (Human); 12406 (Mouse); 29345 (Rat)
  • Alternate Names:
    (collagen binding protein 1); Arsenic-transactivated protein 3; AsTP3; BERF-1; CBP1; CBP2; CBP2RA-A47; Cell proliferation-inducing gene 14 protein; Collagen-binding protein; colligen; Colligin; colligin-1; colligin-2; gp46; HSP47; HSP47serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47); member 1, (collagen binding protein 1); member 2; member 2, (collagen-binding protein 2); OI10; PPROM; proliferation-inducing gene 14; RA-A47; rheumatoid arthritis antigen A-47,47 kDa heat shock protein; Rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47); Serpin H1; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1; SERPINH1; SERPINH2colligin
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