Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

ELISA Detection

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Iduronate 2-Sulfatase/IDS Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IDS (R&D Systems, Catalog # 2449-SU)
Ser26-Pro550
Accession # P22304

Specificity

Detects human Iduronate 2-Sulfatase (IDS) in Western blots. In this format, approximately 50% cross‑reactivity with recombinant mouse IDS is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Iduronate 2‑Sulfatase/IDS Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Iduronate 2-Sulfatase/IDS (Catalog # 2449-SU)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Iduronate 2-Sulfatase/IDS

As a member of the sulfatase family, Iduronate 2-Sulfatase (IDS) is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and dermatan sulfate (2, 3). It hydrolyzes the 2-sulfate group of the L-iduronate 2-sulfate units of the GAG. The IDS deficiency results in mucopolysaccharidosis II (MPS II or Hunter syndrome), an X-linked inborn error leading to lysosomal accumulation of the GAG and its excretion in urine. MPS II has a wide spectrum of clinical manifestations ranging from mild to severe. The deduced amino acid sequence of human IDS consists of a signal peptide (residues 1 - 25), a pro peptide (residues 26 ‑ 33) and a mature chain (residues 34 - 550) that may be further processed into the 42 kDa chain (residues 34 - 455) and the 14 kDa chain (residues 456 - 550) (1).

References

  1. Wilson, P.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8531.
  2. Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
  3. Neufeld, E.F. and Muenzer, J. (2001) in The Metabolic and Molecular Basis of Inherited Disease, Scriver, C.R. et al. (eds.) pp. 3421 - 3452, New York, McGraw-Hill.

Alternate Names

SIDS

Entrez Gene IDs

3423 (Human); 15931 (Mouse)

Gene Symbol

IDS

UniProt

P22304

Additional Iduronate 2-Sulfatase/IDS Products

Product Documents for Human Iduronate 2‑Sulfatase/IDS Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Iduronate 2‑Sulfatase/IDS Biotinylated Antibody

For research use only

Citations for Human Iduronate 2‑Sulfatase/IDS Biotinylated Antibody

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