Human Iduronate 2-Sulfatase/IDS Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2449
Product Details
Citations (1)
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Human Iduronate 2-Sulfatase/IDS Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Iduronate 2-Sulfatase (IDS) in Western blots. In this format, approximately 50% cross‑reactivity with recombinant mouse IDS is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IDS (R&D Systems, Catalog # 2449-SU)
Ser26-Pro550
Accession # P22304
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Iduronate 2-Sulfatase/IDS (Catalog # 2449-SU)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Iduronate 2-Sulfatase/IDS

As a member of the sulfatase family, Iduronate 2-Sulfatase (IDS) is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and dermatan sulfate (2, 3). It hydrolyzes the 2-sulfate group of the L-iduronate 2-sulfate units of the GAG. The IDS deficiency results in mucopolysaccharidosis II (MPS II or Hunter syndrome), an X-linked inborn error leading to lysosomal accumulation of the GAG and its excretion in urine. MPS II has a wide spectrum of clinical manifestations ranging from mild to severe. The deduced amino acid sequence of human IDS consists of a signal peptide (residues 1 - 25), a pro peptide (residues 26 ‑ 33) and a mature chain (residues 34 - 550) that may be further processed into the 42 kDa chain (residues 34 - 455) and the 14 kDa chain (residues 456 - 550) (1).

References
  1. Wilson, P.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8531.
  2. Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
  3. Neufeld, E.F. and Muenzer, J. (2001) in The Metabolic and Molecular Basis of Inherited Disease, Scriver, C.R. et al. (eds.) pp. 3421 - 3452, New York, McGraw-Hill.
Entrez Gene IDs
3423 (Human); 15931 (Mouse)
Alternate Names
Alpha-L-iduronate sulfate sulfatase; EC 3.1.6.13; IDS; iduronate 2-sulfatase 14 kDa chain; iduronate 2-sulfatase 42 kDa chain; iduronate 2-sulfatase; idursulfase; MPS2; S; SIDS

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Citation for Human Iduronate 2-Sulfatase/IDS Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Iduronate-2-sulfatase transport vehicle rescues behavioral and skeletal phenotypes in a mouse model of Hunter syndrome
    Authors: A Arguello, R Meisner, ER Thomsen, HN Nguyen, R Ravi, J Simms, I Lo, J Speckart, J Holtzman, TM Gill, D Chan, Y Cheng, CL Chiu, JC Dugas, M Fang, IA Lopez, H Solanoy, B Tsogtbaata, Y Zhu, A Bhalla, KR Henne, AG Henry, A Delucchi, S Costanzo, JM Harris, D Diaz, K Scearce-Le, PE Sanchez
    JCI Insight, 2021-10-08;6(19):.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Detection

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