Human IFITM1 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF4827V Datasheet / COA / SDS

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AF4827V-100UG

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All IFITM1 Antibodies

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Human IFITM1 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IFITM1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human (rh) IFITM2 and rhIFITM3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IFITM1
Met1-His36
Accession # NP_003632

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: IFITM1

IFITM1 (Interferon inducible transmembrane protein 1; also Leu13, CD225 and "9‑27") is a 17 kDa member of the CD225 family of proteins. It is inducible in multiple cell types by both type I and II interferons, and although reported to occur on the cell surface, is apparently concentrated in the ER. Homotypic IFITM1 interactions promote p53 antiproliferative activity by blocking p53 phosphorylation on Thr55. Human IFITM1 is a 2‑transmembrane protein 125 amino acids (aa) in length. The topology of the molecule is unclear, but transmembrane segments are reported between aa 37‑57 and 87‑107. Over aa 1‑36, human IFITM1 shares only 47% aa identity with mouse IFITM1.

Long Name

Interferon-induced Transmembrane Protein 1

Entrez Gene IDs

8519 (Human); 293618 (Rat)

Alternate Names

CD225 antigen; CD225; CD225Leu-13 antigen; IFI17; IFI17LEU13,9-27; IFITM1; interferon induced transmembrane protein 1 (9-27); Interferon-induced protein 17; interferon-induced transmembrane protein 1; Interferon-inducible protein 9-27; Leu-13