Human IGF-II R/IGF2R Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2447
Detection of IGF-II R/IGF2R in Human Monocytes by Flow Cytometry.
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Human IGF-II R/IGF2R Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGF-II R/IGF2R in ELISAs and Western blots. In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant human (rh) IGF-I R, rhIGF-I, and rhIGF-II is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGF-II R/IGF2R
Ser1510-Phe2108
Accession # P11717
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IGF-II R/IGF2R (Catalog # 2447-GR)
Flow Cytometry
2.5 µg/106 cells
See below

Human IGF-II R/IGF2R Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human IGF-II R/IGF2R Antibody (Catalog # MAB24471)

Standard: Recombinant Human IGF-II R/IGF2R Protein, CF (Catalog # 2447-GR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IGF-II R/IGF2R antibody in Human Monocytes antibody by Flow Cytometry. View Larger

Detection of IGF-II R/IGF2R in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Human IGF-II R/IGF2R Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2447, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-II R/IGF2R

The type 2 insulin-like growth factor receptor (also known as cation-independent mannose-6 phosphate receptor/CI-MPR) is a 300 kDa member of the P-type lectin family of molecules. P-type lectins generate functional eukaryotic lysosomes by binding and sorting lysosomal enzymes expressing phosphorylated mannose residues (M6P) (1‑3). IGF-II R is a type I transmembrane glycoprotein that contains a 2,264 amino acid (aa) extracellular region, a 23 aa transmembrane segment and a 124 aa cytoplasmic tail (4, 5). The extracellular region consists of 15 contiguous “binding” repeats of about 150 aa each. The odd-numbered repeats interact with “ligands” while the even-numbered repeats likely generate a nondisulfide homodimer in the membrane (1). Repeat #11 binds IGF-II, while repeats 3 and 9 bind mannose-6 phosphate; repeat #13 contains a fibronectin type II motif and assists in IGF-II binding (1, 2). In the extracellular region of IGF-II R expressed by R&D Systems (600 aa’s), human IGF-II R is 85% aa identical to both mouse and bovine IGF-II R. This expressed region includes binding repeats #11, 12, and 13. In addition to IGF-II, CI-MPR/IGF-II R binds non-M6P containing ligands such as retinoic acid, urokinase-type plasminogen-activator receptor and plasminogen, plus M6P‑containing molecules such as lysosomal enzymes, TGF-beta 1 precursor, proliferin, LIF, CD26, herpes simplex glycoprotein D and granzymes A and B (2, 6). IGF-II R regulates many diverse biological functions that range from intracellular trafficking to the internalization of extracellular factors and modulation of cellular responses. It delivers newly synthesized M6P‑tagged lysosomal enzymes from the trans-golgi network to endosomes, and facilitates the clearance of extracellular lysosomal and matrix degrading enzymes by internalization into clathrin-coated vesicles and delivery into endosomes. With respect to IGF-II biology, it would appear that IGF‑II R is principally a regulator of local IGF-II levels, targeting IGF-II for destruction in lysosomes (2). However, some evidence suggests the receptor will signal via G‑proteins, an effect that has yet to be conclusively shown (6).

References
  1. Ghosh, P. et al. (2003) Nat. Rev. Mol. Cell. Biol. 4:202.
  2. Dahms, N.M. and M.K. Hancock (2002) Biochim. Biophys. Acta. 1572:317.
  3. Zaina, S. and J. Nilsson (2003) Curr. Opin. Lipidol. 14:483.
  4. Morgan, D.O. et al. (1987) Nature 329:301.
  5. Oshima, A. et al. (1988) J. Biol. Chem. 263:2553.
  6. Hawkes, C. and S. Kar (2004) Brain Res. Rev. 44:117.
Long Name
Insulin-like Growth Factor II Receptor
Entrez Gene IDs
3482 (Human)
Alternate Names
300 kDa mannose 6-phosphate receptor; cation-independent mannose-6 phosphate receptor; cation-independent mannose-6-phosphate receptor; CD222 antigen; CD222; CI Man-6-P receptor; CIMPR; CI-MPR; IGF2R; IGF-II R; IGF-II receptor; IGFIIR; IGF-IIR; insulin-like growth factor 2 receptorM6P/IGF2R; Insulin-like growth factor II receptor; M6P/IGF2 receptor; M6PR; M6P-R; MPR 300; MPR1; MPRI; MPRIM6PR

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Citation for Human IGF-II R/IGF2R Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CD44(+) fibroblasts increases breast cancer cell survival and drug resistance via IGF2BP3-CD44-IGF2 signalling
    Authors: Y Liu, C Yu, Y Wu, X Sun, Q Su, C You, H Xin
    J. Cell. Mol. Med., 2017-05-18;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)

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