Human IGFBP-2 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DGB200
Control Products Available
Human IGFBP-2 ELISA Standard Curve
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Product Details
Procedure
Citations (3)
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Human IGFBP-2 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
Sensitivity
0.09 ng/mL
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human IGFBP-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human IGFBP-2 Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGFBP-2 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human IGFBP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGFBP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IGFBP-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 1.96 6.24 12.7 2.24 6.93 13.6
Standard Deviation 0.07 0.31 0.63 0.17 0.31 0.62
CV% 3.6 5 5 7.6 4.5 4.6

Recovery

The recovery of IGFBP-2 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 100 88-113
Urine (n=4) 109 94-117

Linearity

To assess the linearity of the assay, samples containing high concentrations of IGFBP-2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IGFBP-2 ELISA Linearity

Data Examples

Human IGFBP-2 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGFBP-2

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation.

Long Name:
Insulin-like Growth Factor Binding Protein 2
Entrez Gene IDs:
3485 (Human); 16008 (Mouse)
Alternate Names:
BP2; IBP2; IBP-2; IGF-binding protein 2; IGFBP2; IGFBP-2; IGF-BP53; insulin-like growth factor binding protein 2 (36kD); insulin-like growth factor binding protein 2, 36kDa; insulin-like growth factor-binding protein 2
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IGFBP-2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. CD44 expression in endothelial colony-forming cells regulates neurovascular trophic effect
    Authors: S Sakimoto, V Marchetti, E Aguilar, K Lee, Y Usui, S Murinello, F Bucher, JK Trombley, R Fallon, R Wagey, C Peters, EL Scheppke, PD Westenskow, M Friedlande
    JCI Insight, 2017;2(2):e89906.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Temporal profiles of plasma proteome during childhood development
    Authors: Qibin Zhang
    J Proteomics, 2016;152(0):321-328.
    Species: Human
    Sample Types: Plasma
  3. Chronic hyperglycemia induces trans-differentiation of human pancreatic stellate cells and enhances the malignant molecular communication with human pancreatic cancer cells.
    Authors: Kiss K, Baghy K, Spisak S, Szanyi S, Tulassay Z, Zalatnai A, Lohr J, Jesenofsky R, Kovalszky I, Firneisz G
    PLoS ONE, 2015;10(5):e0128059.
    Species: Human
    Sample Types: Cell Culture Supernates

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