Human IL-1 RII Quantikine ELISA Kit

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Catalog # Availability Size / Price Qty
DR1B00
Control Products Available
Human IL-1 RII ELISA Standard Curve
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Product Details
Procedure
Citations (16)
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Human IL-1 RII Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
Sensitivity
10 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human IL-1 sRII
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human IL-1 sRII Immunoassay is a 3.5 hour solid phase ELISA designed to measure IL-1 sRII in serum, plasma, and cell culture supernates. It contains insect cell-expressed recombinant human IL-1 sRII and has been shown to quantitate the recombinant factor accurately. Results obtained using natural IL-1 sRII showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 sRII. The presence of high levels of IL-1 beta (> 7.5 ng/mL) in samples has been found to produce some interference (> 10% change) when assaying samples containing IL-1 sRII in the mid-level range.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 72.6 371 721 76.1 371 724
Standard Deviation 2.5 7.4 16.9 4.5 17.3 28.5
CV% 3.4 2 2.3 5.9 4.7 3.9

Recovery

The recovery of IL-1 sRII spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 95 86-102
Citrate Plasma (n=5) 101 92-109
EDTA Plasma (n=5) 101 95-107
Heparin Plasma (n=5) 100 91-110
Serum (n=5) 95 87-106

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of IL-1 sRII in various matrices and diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-1 RII ELISA Linearity

Scientific Data

Human IL-1 RII ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 RII

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and, endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex.

IL-1 RII has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.

Long Name:
Interleukin 1 Receptor II
Entrez Gene IDs:
7850 (Human); 16178 (Mouse)
Alternate Names:
beta; CD121b antigen; CD121b; IL-1 R beta; IL-1 RII; IL1R2; IL1RBCD121 antigen-like family member B; IL-1R-beta; IL1RII; IL-1RII; IL-1RT2; IL-1RT-2; interleukin 1 receptor, type II; Interleukin-1 receptor beta; MGC47725
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  10.   Aspirate and wash 3 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IL-1 RII Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Cardiomyocyte IL-1R2 protects heart from ischemia/reperfusion injury by attenuating IL-17RA-mediated cardiomyocyte apoptosis
    Authors: J Lin, Q Li, T Jin, J Wang, Y Gong, Q Lv, M Wang, J Chen, M Shang, Y Zhao, G Fu
    Cell Death & Disease, 2022-01-27;13(1):90.
    Species: Human
    Sample Types: Serum
  2. IL-1R2 expression in human gastric cancer and its clinical significance
    Authors: M Yuan, L Wang, H Huang, Y Li, X Zheng, Q Shao, J Jiang
    Bioscience Reports, 2021-03-26;0(0):.
    Species: Human
    Sample Types: Serum
  3. Whole blood assay as a model for in vitro evaluation of inflammasome activation and subsequent caspase-mediated interleukin-1 beta release
    Authors: TAT Tran, HW Grievink, K Lipinska, C Kluft, J Burggraaf, M Moerland, D Tasev, KE Malone
    PLoS ONE, 2019-04-08;14(4):e0214999.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. IL-1beta is overexpressed and aberrantly regulated in corticosteroid nonresponders with autoimmune inner ear disease.
    Authors: Pathak S, Goldofsky E, Vivas EX, Bonagura VR, Vambutas A
    J. Immunol., 2011-01-03;186(3):1870-9.
    Species: Human
    Sample Types: Plasma
  5. Fatigue in primary Sj0gren's syndrome--a link to sickness behaviour in animals?
    Authors: Harboe E, Tjensvoll AB, Vefring HK, Goransson LG, Kvaloy JT, Omdal R
    Brain Behav. Immun., 2009-06-26;23(8):1104-8.
    Species: Human
    Sample Types: CSF
  6. In vivo regulation of interleukin 1beta in patients with cryopyrin-associated periodic syndromes.
    Authors: Lachmann HJ, Lowe P, Felix SD, Rordorf C, Leslie K, Madhoo S, Wittkowski H, Bek S, Hartmann N, Bosset S, Hawkins PN, Jung T
    J. Exp. Med., 2009-04-13;206(5):1029-36.
    Species: Human
    Sample Types: Plasma
  7. Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
    Authors: Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A
    Blood, 2007-12-10;111(4):2339-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells.
    Authors: Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC
    Br. J. Pharmacol., 2007-03-12;151(1):115-27.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Soluble interleukin-1 receptor type II, IL-18 and caspase-1 in mild cognitive impairment and severe Alzheimer's disease.
    Authors: Lindberg C, Chromek M, Ahrengart L, Brauner A, Schultzberg M, Garlind A
    Neurochem. Int., 2005-06-01;46(7):551-7.
    Species: Human
    Sample Types: Serum
  10. Levels of soluble cytokine factors in temporomandibular joint effusions seen on magnetic resonance images.
    Authors: Kaneyama K, Segami N, Sun W, Sato J, Fujimura K
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005-04-01;99(4):411-8.
    Species: Human
    Sample Types: Synovial Fluid
  11. IL-1 beta, IL-1 receptor antagonist and soluble type II IL-1 receptor in synovial fluid of patients with temporomandibular disorders.
    Authors: Tominaga K, Habu M, Sukedai M, Hirota Y, Takahashi T, Fukuda J
    Arch. Oral Biol., 2004-06-01;49(6):493-9.
    Species: Human
    Sample Types: Synovial Fluid
  12. Determinants of plasma leptin concentration in postmenopausal women.
    Authors: Brooks-Asplund EM, Tupper CE, Daun JM, Kenney WL, Cannon JG
    Mech. Ageing Dev., 2004-02-01;125(2):117-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. White matter lesions and soluble interleukin-1 receptor type II in CSF from demented and non-demented subjects.
    Authors: Bronge L, Wahlund LO, Brauner A, Basun H, Garlind A
    Neurochem. Int., 2002-10-01;41(4):217-22.
    Species: Human
    Sample Types: CSF
  14. Synthesis of type II interleukin-1 receptors by human corneal epithelial cells but not by keratocytes.
    Authors: Cubitt CL, Lausch RN, Oakes JE
    Invest. Ophthalmol. Vis. Sci., 2001-03-01;42(3):701-4.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Functional genomic analysis in arthritis-affected cartilage: yin-yang regulation of inflammatory mediators by alpha 5 beta 1 and alpha V beta 3 integrins.
    Authors: Attur MG, Dave MN, Clancy RM, Patel IR, Abramson SB, Amin AR
    J. Immunol., 2000-03-01;164(5):2684-91.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Estrogen’s bone-protective effects may involve differential IL-1 receptor regulation in human osteoclast-like cells
    Species: Human
    Sample Types: Cell Culture Supernates

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