Human IL-1 Rrp2/IL-1 R6 Antibody

Name: Human IL-1 Rrp2/IL-1 R6 Antibody
Brand: R&D Systems
SKU: MAB872

Catalog #: MAB872 Datasheet / COA / SDS

Discontinued Product

MAB872 has been discontinued.

View all IL-1Rrp2/IL-1R6 products.

Detection of Human IL‑1 Rrp2/IL‑1 R6 by Western Blot.

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All IL-1Rrp2/IL-1R6 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-1 Rrp2/IL-1 R6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑1 Rrp2/IL‑1 R6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant rat IL-1 Rrp2, recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-18 R, rhIL-18 RAcp, and rhIL-18 AcpLR is observed.

Source

Monoclonal Mouse IgG₁ Clone # 116015

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL‑1 Rrp2/IL‑1 R6
Asp20-Tyr337
Accession # Q9HB29

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IL‑1 Rrp2/IL‑1 R6 by Western Blot. Western blot shows lysates of IMR-90 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-1 Rrp2/IL-1 R6 Monoclonal Antibody (Catalog # MAB872) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-1 Rrp2/IL-1 R6 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9 (with 2% milk instead of BSA in the Antibody Solution).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1Rrp2/IL-1R6

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL‑1 R4), IL‑18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). Human IL-1 Rrp2 cDNA encodes a 561 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 318 aa extracellular domain. It shares 67% and 65% amino acid sequence identity with rat and mouse IL 1 Rrp2, respectively. IL-1 Rrp2 is expressed in lung epitheium, brain vasculature, kidney, testis, onocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (2, 3). IL-1 Rrp2 has been shown to mediate the activation of the transcription factor NF kappa B by the IL-1 family ligands IL-1 F6, F8 or F9 (also known as IL-1 epsilon ), with IL-1RAcP as a co‑factor (3, 4). Response to IL-1 F9 is specifically antagonized by IL‑1 F5 (also known as IL-1δ), an IL-1 family ligand that is most closely related to IL-1ra (3). IL-1 Rrp2, IL‑1 F5, and IL-1 F9 are all up-regulated in lesional psoriasis skin, suggesting that the IL-1 Rrp2 mediated signaling pathway may take part in local inflammatory responses (3).

References

Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
Lovenberg, T. W. et al. (1996) J. Neuroimmunol. 70:113.
Debets, R. et al. (2001) J. Immunol. 167:1440.
Towne, J. E. et al. (2004) J. Biol. Chem. 279:13677.

Long Name

Interleukin 1 Receptor-like 2

Entrez Gene IDs

8808 (Human); 107527 (Mouse); 171106 (Rat)

Alternate Names

IL-1 R6; IL-1 Rrp2; IL1RL2; IL-1RL2; IL1Rrp2; IL-1Rrp2; IL1RRP2IL1R-rp2Interleukin-1 receptor-related protein 2; interleukin 1 receptor-like 2; interleukin-1 receptor-like 2