Human IL-12 p70 Antibody Summary
Arg23-Ser219 of p35; Ile23-Ser328 of p40
Accession # P29459 (p35) & P29460 (p40)
Applications
Human IL-12 p70 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL‑12 p70 in Human PBMCs. IL-12 p70 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-12 p70 Monoclonal Antibody (Catalog # MAB611) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-12 p70
Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotrophic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN-gamma inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells. Current evidence indicates that IL-12, produced by macrophages in response to infectious agents, is a central mediator of the cell-mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.
Product Datasheets
Citations for Human IL-12 p70 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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Post-acute sequelae of SARS-CoV-2 cardiovascular symptoms are associated with trace-level cytokines that affect cardiomyocyte function
Authors: Sinclair, JE;Vedelago, C;Ryan, FJ;Carney, M;Redd, MA;Lynn, MA;Grubor-Bauk, B;Cao, Y;Henders, AK;Chew, KY;Gilroy, D;Greaves, K;Labzin, L;Ziser, L;Ronacher, K;Wallace, LM;Zhang, Y;Macauslane, K;Ellis, DJ;Rao, S;Burr, L;Bain, A;Karawita, A;Schulz, BL;Li, J;Lynn, DJ;Palpant, N;Wuethrich, A;Trau, M;Short, KR;
Nature microbiology
Species: Human
Sample Types: Plasma
Applications: Bioassay -
Histamine regulates actin cytoskeleton in human Toll like receptor 4 activated monocyte derived dendritic cells tuning CD4+ T lymphocyte response
J Biol Chem, 2016-05-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
TLR3 and Rig-like receptor on myeloid dendritic cells and Rig-like receptor on human NK cells are both mandatory for production of IFN-gamma in response to double-stranded RNA.
Authors: Perrot I, Deauvieau F, Massacrier C
J. Immunol., 2010-07-16;185(4):2080-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Authors: Pfleger C, Schloot N, ter Veld F
J. Immunol. Methods, 2007-10-22;329(1):214-8.
Species: Human
Sample Types: Serum
Applications: Luminex Development -
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood, 2007-02-08;109(10):4272-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS, 2007;21(10):1263-72.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Clinical improvement in chronic plaque-type psoriasis lesions after narrow-band UVB therapy is accompanied by a decrease in the expression of IFN-gamma inducers -- IL-12, IL-18 and IL-23.
Authors: Piskin G, Tursen U, Sylva-Steenland RM, Bos JD, Teunissen MB
Exp. Dermatol., 2004-12-01;13(12):764-72.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Authors: Copeland S, Siddiqui J, Remick D
J. Immunol. Methods, 2004-01-01;284(1):99-106.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Array Development
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Used as the capture antibody in a sandwich ELISA targeting IL-12p70.
