Human IL-12 R beta 1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB839A-100
FAB839A-025

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Detection of IL‑12 R beta 1 in Daudi cells by Flow Cytometry.
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Human IL-12 R beta 1 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-12 R beta 1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-12 R beta 1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 69310
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta 1
Cys24-Glu540
Accession # P42701
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of IL‑12 R beta 1 in Daudi cells by Flow Cytometry. Daudi cells were stained with Mouse Anti-Human IL‑12 R beta 1 APC‑conjugated Monoclonal Antibody (Catalog # FAB839A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of IL‑12 R beta 1 in PBMC cells by Flow Cytometry. PBMC cells treated with 5ug/ml PHA (Sigma) for 48-72 hours were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human IL‑12 R beta 1 APC‑conjugated Monoclonal Antibody (Catalog # FAB839A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-12 R beta 1

Interleukin12 (IL-12) is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of two subunits designated IL-12 R beta 1 and IL-12 R beta 2. Individually, IL-12 R beta 1 and IL-12 R beta 2 bind IL‑12 with low affinity. Co-expression of both subunits confers high-affinity binding and is required for IL-12 activity. Both IL-12 receptor subunits are type I transmembrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 1 cDNA encodes a 662 amino acid (aa) protein with a putative 23 aa signal peptide that is cleaved to generate the mature protein with a 522 aa extracellular domain, a 25 aa transmembrane domain and a 92 aa cytoplasmic region. Expression of IL-12 R beta 1 is detected in activated T cells, NK cells and B cells. The expression of IL‑12 R beta 2 is more restricted and appears to be limited to Th2 cells.

References
  1. Gately, M.K. et al. (1998) Annu. Rev. Immunol. 16:495.
Long Name
Interleukin 12 Receptor beta 1
Entrez Gene IDs
3594 (Human); 16161 (Mouse)
Alternate Names
CD212 antigen; CD212; IL-12 R beta 1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; IL12R beta 1; IL-12R subunit beta-1; IL12R; IL12RB; IL12RB1; IL-12Rb1; IL-12R-BETA1; IL-12R-beta-1; interleukin 12 receptor, beta 1; interleukin-12 receptor beta-1 chain; interleukin-12 receptor subunit beta-1; MGC34454

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