Human IL-13 R alpha 1 Biotinylated Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-13 R alpha 1 in ELISAs and Western blots. In sandwich ELISAs, less than 1% cross-reactivity with recombinant human (rh) IL‑13 R alpha 2, recombinant mouse (rm) IL-13 R alpha, rhIL-4 R, rhIL-5 R alpha, and rhIL-9 R is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 1
    Ala27-Thr343 (Thr130Ile)
    Accession # Q5JSL4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human IL‑13 R alpha 1 Fc Chimera (Catalog # 146-IR)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
    • Human IL-13 R alpha 1 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human IL‑13 R alpha 1 Antibody (Catalog # MAB146)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human IL‑13 R alpha 1 Biotinylated Antibody (Catalog # BAF152)
  • ELISA Standard
     
    Recombinant Human IL-13 R alpha 1 Fc Chimera Protein, CF (Catalog # 146-IR)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑13 R alpha 1 in Human Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Human IL‑13 R alpha 1 Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF152, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-13 R alpha 1

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

The human IL-13 R alpha 1 was originally cloned based on sequence homology to the mouse IL-13 R alpha 1. The IL-13 R alpha 1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region, and a 59 aa residue cytoplasmic tail. Human and mouse IL-13 R alpha 1 share 76% aa sequence identity. The extracellular domain of IL-13 R alpha 1 is also closely related to that of IL‑13 R alpha 2. IL‑13 R alpha 1 has been shown to combine with the IL-4 R alpha to form a high-affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated.

  • References:
    1. Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
    2. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
    3. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
    4. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.
    5. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.
  • Long Name:
    Interleukin 13 Receptor alpha 1
  • Entrez Gene IDs:
    3597 (Human); 16164 (Mouse)
  • Alternate Names:
    bB128O4.2.1 (interleukin 13 receptor, alpha 1); Cancer/testis antigen 19; CD213a1 antigen; CD213a1; CT19; IL-13 R alpha 1; IL13 receptor alpha-1 chain; IL-13 receptor subunit alpha-1; IL13R alpha 1; IL-13R subunit alpha-1; IL13R; IL13RA; IL-13Ra; IL13RA1; IL-13Ra1; IL-13R-alpha-1; interleukin 13 receptor, alpha 1; interleukin-13 receptor subunit alpha-1; NR4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004;173(11):6592-602.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
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MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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