Human IL-17 RC Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-17 RC in Western blots. In Western blots, approximately 30% cross-reactivity with recombinant mouse IL‑17 RC is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL‑17 RC
    Leu21-Ala454
    Accession # NP_116121
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human IL‑17 RC (Catalog # 2269-IL)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑17 RC in K562 Human Cell Line by Flow Cytometry.

K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human IL‑17 RC Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF2269, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17 RC

IL-17 receptor C (IL-17 RC; also known as IL-17 RL and IL-17 Rhom) is an 85-110 kDa member of the IL-17 receptor family. This is one of five families that currently comprise the cytokine receptor superfamily (1-6). At this time, there are five members within the IL-17 receptor family, and these are termed IL-17 RA, B, C, D, and E. Not all receptors appear to bind known members of the IL-17 cytokine family. To date, IL-17 RA is reported to bind IL-17(A), while IL-17 RB is reported to bind IL‑17B and IL-17E (2, 4). Human IL-17 RC is a type I transmembrane glycoprotein that is expressed on a variety of nonhematopoietic cell types. These include endothelial cells (6, 7), chondrocytes and osteoblasts (8), breast and prostatic epithelium (6), and fibroblasts, plus renal tubular epithelium and skeletal muscle cells (8, 9). Full-length IL-17 RC is synthesized as a 791 amino acid (aa) precursor (10, 11). It contains a 20 aa signal sequence, a 518 aa extracellular domain (ECD) (aa 21‑538), a 21 aa transmembrane segment, and a 232 aa cytoplasmic region. Although IL-17 RA has two fibrinogen-like regions in its ECD that contribute to its function, no such architecture has been identified in the ECD of IL-17 RC (12). Based on studies looking at exon deletions, a key ligand-binding site would appear to exist over aa 425-441 (13). The gene for human IL-17 RC contains 19 exons. It is estimated that there are over 90 alternative splice forms, with transmembrane‑containing isoforms predominating (6, 14). The full-length isoform is estimated to occur approximately 10% of the time, while the three most common isoforms, as a group, occur about 50% of the time. Based on limited information, alternative splicing appears to regulate ligand specificity (13). R&D Systems IL‑17 RC corresponds to IL-17 RC isoform # 3, which shows deletions of aa 36-106 and 264-278 relative to the full-length form (10). Over the ECD, IL‑17 RC isoform #3 is 68% aa identical to mouse IL‑17 RC ECD. IL-17 RC is the cognate receptor for IL-17F, and binds IL-17A with similar affinity (13). With IL‑17 RA, it forms a definitive receptor for both IL-17A and IL-17F. The stoichiometry is unclear; it may form a heterodimer with IL-17 RA, or a heterotrimer with a preexisting IL‑17 RA homodimer (4, 9, 13, 15). The heteromeric nature of the receptor may be important given that the predominant form of the IL-17 cytokine is now considered to be an IL-17A:IL-17F heterodimer (4).

  • References:
    1. Gaffen, S.L. et al. (2006) Vitam. Horm. 74:255.
    2. Weaver, C.T. et al. (2007) Annu. Rev. Immunol. 25:821.
    3. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
    4. Shen, F. and S.L. Gaffen (2008) Cytokine 41:92.
    5. You, Z. et al. (2006) Cancer Res. 66:175.
    6. You, Z. et al. (2007) Neoplasia 9:464.
    7. Gerritsen, M.E. et al. (2003) Br. J. Pharmacol. 140:595.
    8. Kokubu, T. et al. (2008) J. Hictochem. Cytochem. 56:89.
    9. Toy, D. et al. (2006) J. Immunol. 177:36.
    10. GenBank Accession # Q96F46.
    11. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
    12. Kramer, J.M. et al. (2007) J. Immunol. 179:6379.
    13. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
    14. Haudenschild, D.R. et al. (2006) Prostate 66:1268.
    15. Kramer, J.M. et al. (2006) J. Immunol. 176:711.
  • Long Name:
    Interleukin 17 Receptor C, Transcript Variant 3
  • Entrez Gene IDs:
    84818 (Human); 171095 (Mouse)
  • Alternate Names:
    FLJ95963; FLJ96005; IL-17 RC; IL-17 receptor C; IL-17 RL; IL17F receptor; IL17RC; IL-17RC; IL17Rhom; IL17RL; IL-17RL; IL17-RL; interleukin 17 receptor C; interleukin-17 receptor C; Interleukin-17 receptor homolog; Interleukin-17 receptor-like protein; MGC10763; zcytoR14
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