Human IL-17B Biotinylated Antibody

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BAM12481
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Human IL-17B Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17B in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IL-17, rhIL-17C, D, E, F, or recombinant mouse IL-17B is observed.
Source
Monoclonal Mouse IgG1 Clone # 174106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-17B
Gln21-Phe180
Accession # Q9UHF5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample

Human IL-17B Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human IL‑17B Antibody (Catalog # MAB1248)

Standard: Recombinant Human IL-17B Protein (Catalog # 1248-IB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17B

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members, have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL‑17 RE, have also been reported (1‑4). The functions of IL-17 RC, D, and E are not known.

Human IL-17B cDNA encodes a 180 aa protein with a putative 20 aa signal peptide (5, 6). Human and mouse IL-17B share 88% amino acid sequence identity. Among IL-17 family members, IL-17B is most closely related to IL-17D, sharing 27% aa sequence homology. IL-17B is expressed highly in spinal cord, and at lower levels in brain, kidney, lung, small intestine, prostate, testes, pancreas, adrenal gland and trachea (5 - 7). Expression of IL-17B has also been detected in chondrocytes in articular cartilage (2). IL-17B binds the IL-17B receptor but not IL-17 R and exhibits bioactivities distinct from those of IL-17 (5, 6).

References
  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
  4. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  5. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
  6. Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
  7. Moore, E.E. et al. (2002) Neuromuscul. Disord. 12:141.
Long Name
Interleukin 17B
Entrez Gene IDs
27190 (Human); 56069 (Mouse)
Alternate Names
Cytokine Zcyto7; cytokine-like protein ZCYTO7; IL17B; IL-17B; IL-17Binterleukin-17 beta; IL20; IL-20interleukin-17B; interleukin 17B; interleukin 20; Interleukin-20; MGC138900; MGC138901; neuronal interleukin-17 related factor; Neuronal interleukin-17-related factor; NIRF; ZCYTO7interleukin-20

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