Human IL‑18/IL‑1F4 Antibody

R&D Systems | Catalog # D044-3

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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Neutralization, Immunoprecipitation

Cited:

Neutralization, Flow Cytometry, Immunocytochemistry, Antibody Array Development, Cell Culture, ELISA Development (Capture), ELISA Development (Detection), ELISA Microarray Development, Functional Assay, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 125-2H
View all IL-18/IL-1F4 Primary Antibodies »

Product Specifications

Immunogen

Human IL-18/IL-1F4

Specificity

This antibody reacts with human IL-18 on Immunoprecipitation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human IL‑18/IL‑1F4 Antibody

Application
Recommended Usage

Immunoprecipitation

5 μg/0.5 μg recombinant human IL-18

Neutralization

Induction of IFN-gamma by KG-1 cell (Human myelomonocyte: ATCC CCL246) in response to the 40 ng/mL recombinant Human IL-18 was neutralized by this antibody.

Human IL-18/IL-1F4 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human IL‑18/IL‑1F4 Biotinylated Antibody (Catalog # D045-6)
  • Standard: Recombinant Human IL-18/IL-1F4 Protein (Catalog # B001-5)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

100 μg IgG in 100 μL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody solution is stable for one year from the date of purchase when stored at -20°C.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

References

  1. Nussbaumer, O., et al., Blood 118, 2743-2751 (2011)
  2. Tu, A., et al., J. Exp. Med. 205, 233-244 (2008)
  3. Wu, C., J. Immunol. 170, 5571-5577 (2003)
  4. Sugawara, S., J. Immunol. 167, 6568-6575 (2001)
  5. Dao, T., et al., Cell Immunol. 173, 230-235 (1996)
  6. Micallef, M., et al., Eur. J. Immunol. 26, 1647-1651 (1996)
  7. Ushio, S., et al., J. Immunol. 156, 4274-4279 (1996)
  8. Okamura, H., et al., Nature 378, 88-91 (1995)

Long Name

Interleukin 18

Alternate Names

IGIF, IL-1F4, IL-1g, IL18

Entrez Gene IDs

3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)

Gene Symbol

IL18

Additional IL-18/IL-1F4 Products

Product Documents for Human IL‑18/IL‑1F4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL‑18/IL‑1F4 Antibody

For research use only

Citations for Human IL‑18/IL‑1F4 Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Human IL‑18/IL‑1F4 Antibody

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  • Q: I would like to enquire regarding the epitopr of 125-2H mAb. Do you know if it binds total, free or bound IL-18?

    A: We have not mapped the eptiope of the antibody and so we cannot be sure where the antibody binds. It appears it will see all forms but I cannot be absolutely positive.
    Since the immunogen is:
    Immunogen Human IL-18/IL-1F4
    Specificity This antibody reacts with human IL-18 on Immunoprecipitation.

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